鉴定兰花根腐病菌（Rhizoctonia solani）菌系，包括 AG-12，一种新的亲和群。

Characterization of Mycorrhizal Isolates of Rhizoctonia solani from an Orchid, Including AG-12, a New Anastomosis Group.

出版信息

Phytopathology. 1999 Oct;89(10):942-6. doi: 10.1094/PHYTO.1999.89.10.942.

DOI:10.1094/PHYTO.1999.89.10.942

Abstract

ABSTRACT Isolates of Rhizoctonia solani collected from mycorrhizal orchid (Pterostylis acuminata) plants and adjacent leaf litter were characterized. Of 23 selected isolates, 20 were members of a new anastomosis group (AG-12) and the rest were members of AG-6. There were no bridging anastomosis reactions observed between AG-12 and other AGs of R. solani. Among the 20 isolates of AG-12 evaluated, 18 vegetatively compatible populations were detected, indicating diversity within the AG. Mature cultures were dark brown, as were mature sclerotia. Some cultures produced alternating dark- and light-colored concentric rings, with sclerotia forming in the darker rings. Most cultures were appressed to the agar surface. In tests run to characterize pathogenic potential, selected mycorrhizal isolates of AG-12 and AG-6 did little damage to potato and barley seedlings, moderate damage to head lettuce seedlings, and more extensive damage to seedlings of cauliflower and radish. Isolates of AG-12 have not been observed to fruit in nature, and all attempts to induce formation of the teleomorph (Thanatephorus cucumeris) in the laboratory by selected isolates of AG-12 failed.

摘要

摘要从共生兰花（Pterostylis acuminata）植物及其相邻凋落物中分离出的茄丝核菌（Rhizoctonia solani）菌株进行了特征描述。在 23 个选定的菌株中，有 20 个是一个新的吻合群（AG-12）的成员，其余的则是 AG-6 的成员。在 AG-12 和茄丝核菌的其他吻合群之间没有观察到桥接吻合反应。在所评估的 20 个 AG-12 菌株中，检测到 18 个营养体相容种群，表明该吻合群内存在多样性。成熟培养物呈深棕色，与成熟的菌核颜色相同。一些培养物产生交替的深、浅色同心环，菌核在较暗的环中形成。大多数培养物紧贴琼脂表面。在特征致病性潜力的测试中，选定的 AG-12 和 AG-6 共生菌对马铃薯和大麦幼苗的损伤较小，对生菜幼苗的损伤中等，对花椰菜和萝卜幼苗的损伤较大。尚未观察到 AG-12 的菌株在自然界中结出果实，并且通过选择的 AG-12 菌株在实验室中诱导其形成同宗配合体（Thanatephorus cucumeris）的所有尝试均告失败。