Phytopathology. 1999 Mar;89(3):233-8. doi: 10.1094/PHYTO.1999.89.3.233.
ABSTRACT The course of the infection and development of the biotrophic fungus Melampsora larici-populina on leaf tissue from the hybrid poplar Populus deltoides x P. nigra 'Ogy' was monitored at the histological level. Leaf disks were inoculated with one of two rust physiological races (E1 and E2), resulting in interactions that were either incompatible (race E1) or compatible (race E2). In the compatible interaction, the fungus rapidly colonized the leaf without inducing any apparent host response. Symptoms appeared on the leaf several days after inoculation just prior to spore dissemination. The incompatible interaction was characterized by the early collapse and disorganization of cytoplasm of infected cells 17 h after inoculation and within 2 h after the appearance of the first haustoria. Resistance to M. larici-populina was mediated through a hypersensitive response, since it was extremely localized and involved only the few cells that were in the immediate vicinity of each infected cell.
摘要 在组织学水平上监测了生物寄生菌 Melampsora larici-populina 在杂交杨树 Populus deltoides x P. nigra 'Ogy' 叶片组织上的感染和发展过程。将叶片圆盘用两种锈菌生理小种（E1 和 E2）之一进行接种，导致了不亲和（小种 E1）或亲和（小种 E2）的相互作用。在亲和相互作用中，真菌迅速定植于叶片而不诱导任何明显的宿主反应。接种后数天，在孢子传播之前，叶片上出现症状。不亲和相互作用的特征是，在接种后 17 小时和出现第一个吸器后 2 小时内，受感染细胞的细胞质早期崩溃和组织紊乱。对 Melampsora larici-populina 的抗性是通过过敏反应介导的，因为它非常局限，只涉及每个受感染细胞附近的少数几个细胞。
