Phytopathology. 1998 Oct;88(10):1068-72. doi: 10.1094/PHYTO.1998.88.10.1068.
ABSTRACT A gene-for-gene interaction was previously suggested by mapping of a single major locus (LEM 1) controlling cotyledon resistance to Leptosphaeria maculans isolate PHW1245 in Brassica napus cv. Major. In this study, we obtained further evidence of a gene-for-gene interaction by studying the inheritance of the corresponding avirulence gene in L. maculans isolate PHW1245. The analysis of segregating F(1) progenies and 14 test crosses suggested that a single major gene is involved in the interaction. This putative avirulence gene was designated alm1 after the resistance locus identified in B. napus. Amplified fragment length polymorphism (AFLP) markers were used to generate a rudimentary genetic linkage map of the L. maculans genome and to locate markers linked to the putative avirulence locus. Two flanking AFLP markers, AC/TCC-1 and AC/CAG-5, were linked to alm1 at 3.1 and 8.1 cM, respectively. Identification of markers linked to the avirulence gene indicated that the differential interaction is controlled by a single gene difference between parental isolates and provides further support for the gene-for-gene relationship in the Leptosphaeria-Brassica system.
摘要 先前通过定位控制油菜品种植株对 PHW1245 菌系的抗子叶性的单个主效基因（LEM1），提出了基因对基因互作的假说。本研究通过研究 L. maculans 菌系 PHW1245 中相应无毒基因的遗传，进一步证明了基因对基因的互作。对分离的 F1 后代和 14 个测交的分析表明，该互作涉及单个主效基因。该无毒基因被命名为 alm1，该基因与在油菜中鉴定到的抗性基因同源。扩增片段长度多态性（AFLP）标记被用于生成 L. maculans 基因组的初步遗传连锁图谱，并定位与假定无毒基因座连锁的标记。两个侧翼 AFLP 标记 AC/TCC-1 和 AC/CAG-5 分别与 alm1 连锁，距离为 3.1 和 8.1cM。与无毒基因连锁的标记的鉴定表明，差异互作受亲本菌系中单个基因差异的控制，进一步支持了 Leptosphaeria-Brassica 系统中的基因对基因关系。
