Single Gene Introgression Lines for Accurate Dissection of the - Pathosystem.

Seven blackleg resistance () genes ( & ) were each introgressed into a common susceptible doubled-haploid (DH) line through reciprocal back-crossing, producing single- gene introgression lines (ILs) for use in the pathological and molecular study of - interactions. The genomic positions of the genes were defined through molecular mapping and analysis with transgenic isolates was used to confirm the identity of the introgressed genes where possible. Using isolates of contrasting avirulence gene () profiles, we preformed extensive differential pathology for phenotypic comparison of the ILs to other varieties, demonstrating the ILs can provide for the accurate assessment of - gene interactions by avoiding non- dependant alterations to resistance responses which can occur in some commonly used varieties. Whole-genome SNP-based assessment allowed us to define the donor parent introgressions in each IL and provide a strong basis for comparative molecular dissection of the pathosystem.