Phytopathology. 1998 Aug;88(8):844-50. doi: 10.1094/PHYTO.1998.88.8.844.
ABSTRACT Forty bacterial strains isolated from leek blight (Allium porrum) in France and other countries were studied by conventional biochemical methods, serological reactions, numerical taxonomy, DNA-DNA hybridization, and ice nucleation activity, as well as by pathogenicity on leek and other host plants. They were compared with reference strains of Pseudomonas, mainly pathotype strains of P. syringae pathovars and strains of P. syringae pv. syringae isolated from various host plants including onions. Leek strains sorted with P. syringae species (sensu lato) by LOPAT tests (production of levan-sucrase, oxidase, pectinase, arginine dihydrolase, and hypersensitive reaction on tobacco). Leek strains were pathogenic to leek and produced symptoms identical to those observed in the field. They were the only strains in our study that could cause blight of leek. Thus, our results justify the creation of a new pathovar. Leek strains constituted a highly homogeneous DNA group and a discrete phenon by numerical taxonomy, and they belonged to O-serogroup POR. The name of P. syringae pv. porri is proposed for the bacterium causing leek blight. Criteria for routine identification are presented and taxonomic status is discussed.
摘要 从法国和其他国家的韭菜疫病（Allium porrum）中分离出的 40 株细菌菌株，通过常规生化方法、血清学反应、数值分类学、DNA-DNA 杂交和冰核活性以及对韭菜和其他宿主植物的致病性进行了研究。并与参考菌株 Pseudomonas 进行了比较，主要是 P. syringae 种的致病性菌株和从各种宿主植物（包括洋葱）中分离出的 P. syringae pv. syringae 菌株。LOPAT 试验（产生纤维二糖-蔗糖酶、氧化酶、果胶酶、精氨酸二氢酶和对烟草的超敏反应）将韭菜菌株归类为 P. syringae 种（广义）。韭菜菌株对韭菜具有致病性，并产生与田间观察到的相同的症状。它们是我们研究中唯一能够引起韭菜疫病的菌株。因此，我们的结果证明了创建一个新的致病变种是合理的。通过数值分类学，韭菜菌株构成了一个高度同源的 DNA 群和一个离散的表型，它们属于 O-血清群 POR。建议将引起韭菜疫病的细菌命名为 P. syringae pv. porri。提出了常规鉴定的标准并讨论了分类地位。
