人乳铁蛋白 cDNA 的表达赋予转基因烟草植株对青枯雷尔氏菌的抗性。
Expression of Human Lactoferrin cDNA Confers Resistance to Ralstonia solanacearum in Transgenic Tobacco Plants.
出版信息
Phytopathology. 1998 Jul;88(7):730-4. doi: 10.1094/PHYTO.1998.88.7.730.
ABSTRACT A construct containing a human lactoferrin cDNA was used to transform tobacco (Nicotiana tabacum) using an Agrobacterium-mediated DNA-transfer system to express this human protein in transgenic plants. Transformants were analyzed by Southern, Northern, and Western blots to determine integration of the cDNA into the plant genome and lactoferrin gene expression levels. Most transgenic plants demonstrated significant delays of bacterial wilt symptoms when inoculated with the bacterial pathogen Ralstonia solanacearum. Quantification of the expressed lactoferrin protein by enzyme-linked immunosorbent assay in transgenic plants indicated a significant positive relationship between lactoferrin gene expression levels and levels of disease resistance. Incorporation of the lactoferrin gene into crop plants may enhance resistance to other phytopathogenic bacteria as well.
摘要
使用含有人乳铁蛋白 cDNA 的构建体，通过农杆菌介导的 DNA 转移系统将其转化烟草（Nicotiana tabacum），以在转基因植物中表达这种人蛋白。通过 Southern、Northern 和 Western 印迹分析来确定 cDNA 是否整合到植物基因组中以及乳铁蛋白基因的表达水平。当用细菌病原体青枯菌接种时，大多数转基因植物的细菌性萎蔫症状出现明显延迟。通过酶联免疫吸附试验在转基因植物中对表达的乳铁蛋白蛋白进行定量分析表明，乳铁蛋白基因表达水平与疾病抗性水平之间存在显著的正相关关系。将乳铁蛋白基因整合到农作物中可能也会增强对其他植物病原菌的抗性。
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