Phytopathology. 1998 Feb;88(2):156-63. doi: 10.1094/PHYTO.1998.88.2.156.
ABSTRACT Complete cosegregation for race-specific incompatibility with three Melampsora larici-populina rust races was observed in five F(1) hybrid progenies of Populus, with different patterns among the various progenies. A single gene cluster could explain these segregations: one locus with multiple alleles or two tightly linked loci controlling complete resistance to E1 and E3, and two tightly linked loci for E2. The random amplified polymorphic DNA marker OPM03/04_480 was linked to that cluster in all families (<1 cM). This marker accounted for more than 70% of the genetic variation for field resistance in each family (heritability approximately 0.40). The same marker accounted for up to 64% of the clonal variation for growth in the nursery under natural inoculum pressure; the weak tolerance to rust of F(1) interspecific hybrids was attributed to a genetic background effect. Partial resistance was split into epidemiological components (heritability ranged from 0.35 to 0.87). Genotypic correlations among resistance traits for the different races were high (0.73 to 0.90). However, correlations among different resistance components for a single race were not all significant. A major quantitative trait locus for all components of partial resistance to E2 was associated to the cluster controlling incompatibility to E1 and E3 and marked by OPM03/04_480 (R(2)from 48 to 68%).
摘要 在杨树的五个 F1 杂种后代中，观察到与三种落叶松杨栅锈菌锈菌特定不相容性的完全连锁，不同后代的模式也不同。一个单一的基因簇可以解释这些分离：一个具有多个等位基因的位点或两个紧密连锁的位点控制对 E1 和 E3 的完全抗性，以及两个紧密连锁的位点控制对 E2 的抗性。随机扩增多态性 DNA 标记 OPM03/04_480 与所有家族中的该簇连锁（<1 cM）。该标记解释了每个家族中田间抗性遗传变异的 70%以上（遗传力约为 0.40）。同一标记在自然接种压力下的苗圃生长中解释了高达 64%的无性系变异；F1 种间杂种对锈病的弱耐受性归因于遗传背景效应。部分抗性分为流行病学成分（遗传力范围为 0.35 至 0.87）。不同锈菌的抗性性状之间的基因型相关性很高（0.73 至 0.90）。然而，单个锈菌的不同抗性成分之间的相关性并非全部显著。与控制 E1 和 E3 不相容性的簇相关联并由 OPM03/04_480 标记的 E2 部分抗性所有成分的主要数量性状位点（R2 为 48%至 68%）。
