Phytopathology. 1997 Dec;87(12):1184-91. doi: 10.1094/PHYTO.1997.87.12.1184.
ABSTRACT Isolates of Colletotrichum lindemuthianum (138 total) from Argentina, Brazil, the Dominican Republic, Honduras, Mexico, and the United States were characterized into 41 races based on virulence to 12 differential cultivars of Phaseolus vulgaris. These 41 races were categorized into two groups: those found over a wide geographic area and those restricted to a single country. Races 7, 65, and 73 were widespread. Race 73 was the most common (28%). Race 7 was found once in Argentina and Mexico but at a higher frequency in the United States. Race 65 was found repeatedly in Brazil and the United States. Although 39% of the races were detected repeatedly and three races were widespread, no race was isolated from both P. vulgaris gene pools. Phenetic analyses showed no obvious patterns correlated with virulence clusters. No geographic pattern was evident. Molecular polymorphism generated by random amplified polymorphic DNA confirmed the extensive variability in virulence of C. lindemuthianum. Virulence phenotypes were grouped into 15 clusters. The two largest clusters contained isolates from all the geographic regions sampled. Molecular polymorphism was observed among isolates from races 65 and 73 within and among countries, except among Bra-zilian isolates of race 65. The genetic diversity of C. lindemuthianum was greatest in Mexico and Honduras. Our data suggest that C. lindemuthianum may not be highly structured to specific Phaseolus gene pools.
摘要 从阿根廷、巴西、多米尼加共和国、洪都拉斯、墨西哥和美国采集了 138 株炭疽菌（Colletotrichum lindemuthianum）分离物，根据对 12 种不同菜豆（Phaseolus vulgaris）品种的毒力，将其分为 41 个小种。这些小种分为两类：广泛分布的小种和局限于单一国家的小种。小种 7、65 和 73 分布广泛。小种 73 最为常见（28%）。小种 7 仅在阿根廷和墨西哥出现过，但在美国更为常见。小种 65 反复出现在巴西和美国。尽管 39%的小种反复出现，三种小种分布广泛，但没有一种小种同时存在于菜豆的两个基因库中。表型分析显示，毒力聚类与明显的模式无关。没有明显的地理模式。随机扩增多态性 DNA 产生的分子多态性证实了炭疽菌毒力的广泛变异性。毒力表型分为 15 个聚类。两个最大的聚类包含了来自所有采样地理区域的分离物。在国家内部和国家之间观察到小种 65 和 73 的分离物之间存在分子多态性，但巴西小种 65 的分离物除外。炭疽菌的遗传多样性在墨西哥和洪都拉斯最大。我们的数据表明，炭疽菌可能不会高度适应特定的菜豆基因库。
