Phytopathology. 1998 Apr;88(4):292-9. doi: 10.1094/PHYTO.1998.88.4.292.
ABSTRACT Differential cultivars and molecular markers were used to analyze 59 isolates of the bean anthracnose pathogen, Colletotrichum lindemuthianum, from different regions of Mexico. Ten distinct races were determined, three of which had not been reported previously in Mexico. Isolates were found to infect only a narrow range of the differential cultivars used and were restricted to cultivars of Middle American origin. A comparison of random amplified polymorphic DNA and amplified fragment length polymorphism (AFLP) analyses was carried out on a subset of the fungal isolates. Determination of genetic distances based on AFLP data and production of a dendrogram demonstrated two levels of association: i) isolates classified into two major groups according to the type of cultivar or system of cultivation from which they originated, and ii) isolates could be classified into smaller subgroups generally associated with the geographic location from which they were obtained. Bootstrap analysis and determination of confidence intervals showed these geographic groupings to be extremely robust.
摘要 采用不同品种和分子标记对来自墨西哥不同地区的 59 株菜豆炭疽病菌（Colletotrichum lindemuthianum）进行了分析。确定了 10 个不同的小种，其中 3 个以前在墨西哥没有报道过。发现这些分离物仅能感染使用的差异品种的狭窄范围，并且局限于中美洲起源的品种。对真菌分离物的一部分进行了随机扩增多态性 DNA 和扩增片段长度多态性 (AFLP) 分析的比较。根据 AFLP 数据确定遗传距离并生成系统发育树表明存在两个关联水平：i）根据其起源的品种类型或栽培系统将分离物分类为两个主要组，ii）可以将分离物分类为通常与获得它们的地理位置相关的较小亚组。自举分析和置信区间的确定表明，这些地理分组非常稳健。
