Phytopathology. 1997 Dec;87(12):1192-6. doi: 10.1094/PHYTO.1997.87.12.1192.
ABSTRACT Strains of Pseudomonas syringae (78 strains and 43 pathovars) and other strains (79) of plant and insect origin were examined for the presence of the ethylene-forming enzyme gene (efe) by polymerase chain reaction (PCR) assay. The sequence of the efe gene of P. syringae pv. phaseolicola PK2 was used to design two primer sets for amplification of the gene. In addition to P.syringae pv. phaseolicola (the "kudzu strain") and P.syringae pv. glycinea, which were efficient ethylene producers, several strains of P.syringae pvs. sesami and cannabina generated PCR products of the predicted size. A DNA probe of the efe gene, isolated from strain PK2, hybridized to these PCR products, indicating homology to the P.syringae pv. phaseolicola efe gene. PCR restriction fragment length polymorphism analyses suggested that these four pathovars harbor a similar efe gene. Furthermore, the probe hybridized to an indigenous plasmid of P.syringae pv. cannabina, suggesting that the efe gene could be located on a plasmid in this pathovar, but did not hybridize to plas-mids of P.syringae pv. sesami strains. P.syringae pvs. sesami and cannabina strains produced ethylene in King's medium B at levels similar to those of P.syringae pvs. phaseolicola and glycinea. Thus, two new ethylene-producing bacteria were detected by the PCR assay.
摘要 通过聚合酶链反应（PCR）检测了 78 株、43 个血清型的丁香假单胞菌（Pseudomonas syringae）菌株和其他植物及昆虫来源的菌株（79 株）中乙烯形成酶基因（efe）的存在情况。根据丁香假单胞菌 pv.phaseolicola PK2 的 efe 基因序列设计了两对引物，用于扩增该基因。除了高效产生乙烯的丁香假单胞菌 pv.phaseolicola（“葛藤菌株”）和 pv.glycinea 之外，几个 pv.sesami 和 pv.cannabina 血清型的菌株也产生了预期大小的 PCR 产物。从 PK2 菌株中分离出的 efe 基因 DNA 探针与这些 PCR 产物杂交，表明与 pv.phaseolicola 的 efe 基因同源。PCR 限制性片段长度多态性分析表明，这四个血清型携带相似的 efe 基因。此外，该探针与 pv.cannabina 的土著质粒杂交，表明 efe 基因可能位于该血清型的质粒上，但与 pv.sesami 菌株的质粒不杂交。pv.sesami 和 pv.cannabina 菌株在 King 氏 B 培养基中产生的乙烯水平与 pv.phaseolicola 和 pv.glycinea 相似。因此，通过 PCR 检测到了两种新的产乙烯细菌。
