Nagahama K, Yoshino K, Matsuoka M, Sato M, Tanase S, Ogawa T, Fukuda H
Department of Applied Microbial Technology, Kumamoto Institute of Technology, Japan.
Microbiology (Reading). 1994 Sep;140 ( Pt 9):2309-13. doi: 10.1099/13500872-140-9-2309.
The molecular characteristics of the ethylene-forming enzymes of strains of Pseudomonas syringae were tested. The ethylene-producing activities of the nine strains as measured in vivo and in vitro were similar, except for that of P. syringae pv. mori M5. A polyclonal antibody and a DNA probe for the ethylene-forming enzyme from P. syringae pv. phaseolicola PK2 were prepared to investigate homologies among the proteins and genes for the ethylene-forming enzymes. With the exception of P. syringae pv. mori M5, eight strains tested expressed the same antigen as the ethylene-forming enzyme from P. syringae pv. phaseolicola PK2 and were homologous to DNA sequences on indigenous plasmids. Molecular masses of antigenic proteins from all ethylene-producing strains were 40 kDa. The N-terminal amino acid sequence of the purified ethylene-forming enzyme from P. syringae pv. glycinea KN130 was identical to that of the enzyme from P. syringae pv. phaseolicola PK2. These results show that the ethylene-forming enzymes encoded by the indigenous plasmid(s) in the pathogenic bacteria examined were similar.
对丁香假单胞菌菌株的乙烯形成酶的分子特性进行了测试。除丁香假单胞菌 pv. 桑 M5 外，所测的 9 个菌株在体内和体外的乙烯产生活性相似。制备了针对菜豆丁香假单胞菌 PK2 的乙烯形成酶的多克隆抗体和 DNA 探针，以研究乙烯形成酶的蛋白质和基因之间的同源性。除丁香假单胞菌 pv. 桑 M5 外，所测试的 8 个菌株表达的抗原与菜豆丁香假单胞菌 PK2 的乙烯形成酶相同，并且与内源质粒上的 DNA 序列同源。所有产乙烯菌株的抗原蛋白的分子量均为 40 kDa。大豆丁香假单胞菌 KN130 纯化的乙烯形成酶的 N 端氨基酸序列与菜豆丁香假单胞菌 PK2 的该酶相同。这些结果表明，在所检测的病原菌中，由内源质粒编码的乙烯形成酶相似。
