Department of Diagnostic Services, Pathology and Legal Medicine, Section of Legal Medicine, University of Modena and Reggio Emilia, Modena, Italy.
Forensic Sci Int. 2008 Oct 25;181(1-3):21-5. doi: 10.1016/j.forsciint.2008.08.011. Epub 2008 Oct 21.
The comparison of 4 cases of immediate anaphylactic death following the intramuscular injection of antibiotics (different types of penicillin or cephalosporins) with 4 cases of immediate non-anaphylactic death (induced by different causes) recognized splenic eosinophilia, as the main feature for the differential diagnosis, in agreement with isolated previous studies. The use of a stain (pagoda red) little known and seldom employed in Pathology and in Forensic Medicine, showed the concomitant massive presence (immunohistochemically confirmed) of mast cells and degranulated mast cells, the latter mainly located in splenic sinuses. The whole of our findings led us to consider the spleen as the possible shock organ in man. Waiting for further judgment on our hypothesis, we seized the opportunity to remark the employment of the pagoda red stain when the contemporary demonstration of eosinophils, mast cells and degranulated mast cells is required.
对 4 例因肌肉注射抗生素（不同类型的青霉素或头孢菌素）而立即发生过敏死亡的病例和 4 例因不同原因立即发生非过敏死亡的病例进行比较，发现脾嗜酸性粒细胞增多是鉴别诊断的主要特征，这与之前的一些孤立研究结果一致。使用一种在病理学和法医学中鲜为人知且很少使用的染色剂（宝塔红）显示同时存在大量（免疫组织化学证实）肥大细胞和脱颗粒肥大细胞，后者主要位于脾窦内。我们所有的发现使我们认为脾脏可能是人类休克的器官。在等待对我们的假设进行进一步判断的同时，我们抓住机会指出，当需要同时显示嗜酸性粒细胞、肥大细胞和脱颗粒肥大细胞时，可以使用宝塔红染色剂。
