Mukherjee Pranab K, Long Lisa, Kim Hyung Gun, Ghannoum Mahmoud A
Center for Medical Mycology, Department of Dermatology, Case Western Reserve University School of Medicine and University Hospitals of Cleveland, Cleveland, OH 44106-5028, USA.
Int J Antimicrob Agents. 2009 Feb;33(2):149-53. doi: 10.1016/j.ijantimicag.2008.07.030. Epub 2008 Oct 21.
The purpose of this study was to determine the efficacy of amphotericin B lipid complex (ABLC) against Candida albicans biofilms using a rabbit model of catheter-associated candidal biofilm. A clinical C. albicans isolate was allowed to form biofilms on catheters placed in vivo and was then exposed to lock therapy with ABLC (1.5mg, locked for 4h or 8h for 7 days). Untreated biofilms served as controls. Fungal loads on the proximal and distal sections of catheters were determined by counting colony-forming units (CFUs), whilst surface architecture of formed biofilms was evaluated by scanning electron microscopy (SEM). Studies revealed that all ABLC-treated catheters were sterilised and yielded 0 CFU (P<or=0.0027 for both proximal and distal segments compared with the untreated controls). Furthermore, SEM analyses showed that while catheters retrieved from untreated control animals were overlaid with a thick biofilm, those treated with ABLC lock therapy showed only some debris with no fungal cells. Taken together, these studies demonstrated that using ABLC it was possible to sterilise catheters on which C. albicans biofilms were formed.
本研究的目的是利用导管相关念珠菌生物膜的兔模型，确定两性霉素B脂质复合物（ABLC）对白色念珠菌生物膜的疗效。将一株临床分离的白色念珠菌接种于体内留置的导管上形成生物膜，然后用ABLC（1.5mg，锁定4小时或8小时，共7天）进行封管治疗。未治疗的生物膜作为对照。通过计数菌落形成单位（CFU）来确定导管近端和远端的真菌负荷，同时通过扫描电子显微镜（SEM）评估形成的生物膜的表面结构。研究表明，所有接受ABLC治疗的导管均被灭菌，CFU为0（与未治疗的对照组相比，近端和远端节段的P值均≤0.0027）。此外，SEM分析显示，从未治疗的对照动物体内取出的导管上覆盖着一层厚厚的生物膜，而接受ABLC封管治疗的导管上仅显示一些碎片，没有真菌细胞。综上所述，这些研究表明，使用ABLC可以对形成白色念珠菌生物膜的导管进行灭菌。
