Cesaroni Matteo, Cittaro Davide, Brozzi Alessandro, Pelicci Pier Giuseppe, Luzi Lucilla
Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milano, Italy.
Bioinformatics. 2008 Dec 15;24(24):2918-20. doi: 10.1093/bioinformatics/btn542. Epub 2008 Oct 21.
CARPET (collection of automated routine programs for easy tiling) is a set of Perl, Python and R scripts, integrated on the Galaxy2 web-based platform, for the analysis of ChIP-chip and expression tiling data, both for standard and custom chip designs. CARPET allows rapid experimental data entry, simple quality control, normalization, easy identification and annotation of enriched ChIP-chip regions, detection of the absolute or relative transcriptional status of genes assessed by expression tiling experiments and, more importantly, it allows the integration of ChIP-chip and expression data. Results can be visualized instantly in a genomic context within the UCSC genome browser as graph-based custom tracks through Galaxy2. All generated and uploaded data can be stored within sessions and are easily shared with other users.
CARPET(用于轻松拼接的自动化常规程序集合)是一组Perl、Python和R脚本,集成在基于Galaxy2的网络平台上,用于分析ChIP芯片和表达拼接数据,适用于标准芯片设计和定制芯片设计。CARPET允许快速输入实验数据、进行简单的质量控制、标准化、轻松识别和注释富集的ChIP芯片区域、检测通过表达拼接实验评估的基因的绝对或相对转录状态,更重要的是,它允许整合ChIP芯片和表达数据。结果可以通过Galaxy2在UCSC基因组浏览器的基因组背景下即时可视化为基于图形的自定义轨迹。所有生成和上传的数据都可以存储在会话中,并很容易与其他用户共享。