Yun Jun-Won, Shin Eui-Seok, Cho Si-Young, Kim Shin-Hyoung, Kim Chae-Wook, Lee Tae-Ryong, Kim Bae-Hwan
Amorepacific Corporation R&D Center, Daegu, Korea.
Exp Anim. 2008 Oct;57(5):461-9. doi: 10.1538/expanim.57.461.
Adiponectin, which is expressed exclusively in adipose tissue, has been shown to increase fatty acid oxidation via activation of AMP-activated kinase (AMPK) and phosphorylation of acetyl CoA carboxylase (ACC). ACC phosphorylation and carnitine palmitoyl-transferase-1 (CPT1) activity have been shown to be rate controlling factors in fatty acid oxidation. In high fat diet (HFD)-induced obese mice, we analyzed the time-course of changes in the expression of adiponectin and lipid oxidative enzymes induced by treatment with bisphenol A diglycidyl ether (BADGE) or caffeine for 8 weeks, and investigated whether the changes of adiponectin and lipid oxidative enzymes expression correlated with reduced adiposity or steatosis after 8 weeks of treatment. After 8 weeks of treatment, BADGE and caffeine had reduced body weight and epididymal adipose tissue weight in mice fed HFD, and markedly reduced the number of fatty droplets in the liver. Interestingly, the expression of adiponectin and lipid oxidative enzymes significantly increased after 2 weeks of treatment. These results indicate that the expression of adiponectin and lipid oxidative enzymes in the early stages of BADGE or caffeine treatment correlated well with the long-term anti-obesity effects.
脂联素仅在脂肪组织中表达，已被证明可通过激活AMP活化蛋白激酶（AMPK）和乙酰辅酶A羧化酶（ACC）的磷酸化来增加脂肪酸氧化。ACC磷酸化和肉碱棕榈酰转移酶-1（CPT1）活性已被证明是脂肪酸氧化的速率控制因素。在高脂饮食（HFD）诱导的肥胖小鼠中，我们分析了用双酚A二缩水甘油醚（BADGE）或咖啡因处理8周所诱导的脂联素和脂质氧化酶表达变化的时间进程，并研究了脂联素和脂质氧化酶表达的变化是否与8周治疗后肥胖或脂肪变性的减轻相关。治疗8周后，BADGE和咖啡因降低了高脂饮食喂养小鼠的体重和附睾脂肪组织重量，并显著减少了肝脏中的脂肪滴数量。有趣的是，治疗2周后脂联素和脂质氧化酶的表达显著增加。这些结果表明，BADGE或咖啡因治疗早期脂联素和脂质氧化酶的表达与长期抗肥胖作用密切相关。
