Korea Food Research Institute, Bundang-gu, Seongnam, Gyeonggi, Republic of Korea.
Mol Nutr Food Res. 2015 Apr;59(4):784-94. doi: 10.1002/mnfr.201400470. Epub 2015 Mar 3.
Green tea (GT) consumption helps to prevent and control obesity by stimulating hepatic lipid metabolism. However, GT-induced changes in serum and liver metabolomes associated with the anti-obesity effects are not clearly understood. The aim of this study was to identify and validate metabolomic profiles in the livers and sera of GT-fed obese mice to elucidate the relationship between GT consumption and obesity prevention.
Serum and liver metabolites were analyzed in mice fed normal diet, high-fat diet (HFD), HFD with GT, and HFD with crude catechins, using LC-quadrupole TOF MS. The addition of 1% GT to HFD reduced adipose tissue and the levels of blood triglycerides, glucose, insulin, and leptin elevated in HFD-fed mice. We proposed an HFD-induced obesity pathway and validated it by investigating the key regulatory enzymes of mitochondrial β-oxidation: carnitine palmitoyltransferase-1 and -2, acyl-coenzyme A dehydrogenase, and acetyl-coenzyme A acyltransferase. The results showed that HFD-induced abnormal mitochondrial β-oxidation was moderated by the consumption of caffeine- and theanine-enriched GT.
Results of LC/MS-based metabolomic analysis of obese mice showed changes associated with abnormal lipid and energy metabolism, which were alleviated by GT intake, indicating the mechanism underlying the anti-obesity effects of GT.
绿茶（GT）的消费通过刺激肝脏脂质代谢有助于预防和控制肥胖。然而，与抗肥胖作用相关的 GT 诱导的血清和肝脏代谢组学变化尚不清楚。本研究的目的是鉴定和验证 GT 喂养肥胖小鼠肝脏和血清中的代谢组学图谱，以阐明 GT 消费与肥胖预防之间的关系。
使用 LC-四极杆飞行时间 MS 分析正常饮食、高脂肪饮食（HFD）、HFD 加 GT 和 HFD 加儿茶素粗提物喂养的小鼠的血清和肝脏代谢物。将 1% GT 添加到 HFD 中可减少脂肪组织，降低 HFD 喂养小鼠血液中甘油三酯、葡萄糖、胰岛素和瘦素的水平。我们提出了一种 HFD 诱导的肥胖途径，并通过研究线粒体β-氧化的关键调节酶：肉碱棕榈酰转移酶-1 和 -2、酰基辅酶 A 脱氢酶和乙酰基辅酶 A 酰基转移酶来验证它。结果表明，GT 中富含咖啡因和茶氨酸的摄入可调节 HFD 诱导的异常线粒体β-氧化。
肥胖小鼠基于 LC/MS 的代谢组学分析结果显示与异常脂质和能量代谢相关的变化，GT 摄入可减轻这些变化，表明 GT 抗肥胖作用的机制。
