Collins D C, Balikian H M, Preedy J R
Steroids. 1976 Nov;28(5):597-612. doi: 10.1016/0039-128x(76)90002-7.
A loading dose of 3H-estriol was given to male dogs followed by a constant infusion. The concentrations of total radioactivity, conjugated estriol metabolites, estriol, estriol-o-glucosiduronate, estriol-16alpha-glucosiduronate, estriol-3-sulfate and estriol-3-sulfate, 16alpha-glucosiduronate were determined in plasma from the femoral artery(A), hepatic vein(HV) and superior mesenteric vein (SMV). From these values the splanchnic (100[1-HV/A]) and intestinal (100[1-SMV/A]) extractions were calculated. The mean splanchnic extraction of total radioactivity was positive (23, SE 3, P less than .01), indicating net uptake by the splanchnic area, possibly due to biliary excretion. The mean splanchnic extraction of estriol was 77, SE 1, P less than .01, also indicating net uptake. The splachnic extractions of estriol-3-glucosiduronate, estriol-16alpha-glucosiduronate and estriol-3-sulfate were negative (-15, SE 3, P less than .01; -23, SE 6, P less than .01; -31, SE 8, P less than .01 respectively) indicating net formation of these conjugates for release into the systemic circulation. The mean intestinal extraction of estriol was 12, SE 4, P less than .01, indicating net uptake by the intestine. This net uptake was associated with mean negative intestinal extractions of estriol-3-glucosiduronate (-15, SE 7, P approximately .05), estriol-3-sulfate (-33, SE 10, P less than .01) and estriol-3-sulfate, 16alpha-glucosiduronate (-53, SE 13, P less than .01), indicating net formation of these conjugates by the intestine.
给雄性犬静脉注射一剂3H-雌三醇，随后持续输注。测定股动脉（A）、肝静脉（HV）和肠系膜上静脉（SMV）血浆中总放射性、结合型雌三醇代谢物、雌三醇、雌三醇 - o - 葡萄糖醛酸苷、雌三醇 - 16α - 葡萄糖醛酸苷、雌三醇 - 3 - 硫酸盐以及雌三醇 - 3 - 硫酸盐 - 16α - 葡萄糖醛酸苷的浓度。根据这些数值计算内脏（100[1 - HV/A]）和肠道（100[1 - SMV/A]）的提取率。总放射性的平均内脏提取率为正值（23，标准误3，P <.01），表明内脏区域有净摄取，可能是由于胆汁排泄。雌三醇的平均内脏提取率为77，标准误1，P <.01，也表明有净摄取。雌三醇 - 3 - 葡萄糖醛酸苷、雌三醇 - 16α - 葡萄糖醛酸苷和雌三醇 - 3 - 硫酸盐的内脏提取率为负值（分别为 - 15，标准误3，P <.01； - 23，标准误6，P <.01； - 31，标准误8，P <.01），表明这些结合物有净生成并释放到体循环中。雌三醇的平均肠道提取率为12，标准误4，P <.01，表明肠道有净摄取。这种净摄取与雌三醇 - 3 - 葡萄糖醛酸苷（ - 15，标准误7，P约为.05）、雌三醇 - 3 - 硫酸盐（ - 33，标准误10，P <.01）以及雌三醇 - 3 - 硫酸盐 - 16α - 葡萄糖醛酸苷（ - 53，标准误13，P <.01）的平均肠道负提取率相关，表明肠道有这些结合物的净生成。
