给予雌三醇-2,4,6,7-³H后犬血浆中结合雌激素代谢物的鉴定。

Identification of conjugated estrogen metabolites in dog plasma following administration of estriol-2,4,6,7-3H.

作者信息

Collins D C, Musey P I, Preedy J R

出版信息

Steroids. 1976 Jul;28(1):67-80. doi: 10.1016/0039-128x(76)90126-4.

Abstract

Following the constant infusion of 2,4,6,7-3H-estriol in male dogs for a period of 90 minutes, the radioactive metabolites present in arterial plasma were separated by solvent partition, DEAE-Sephadex, Celite partition and thin layer chromatography. The identities of the individual estrogens and estrogen conjugates were confirmed by specific activity determinations after chromatography in several different solvent systems, enzyme hydrolysis and chromatography of the unconjugated steroids and their derivatives.

摘要

在雄性犬中持续输注2,4,6,7-³H-雌三醇90分钟后，通过溶剂分配、DEAE-葡聚糖凝胶、硅藻土分配和薄层色谱法分离动脉血浆中存在的放射性代谢物。在几种不同溶剂系统中进行色谱分析、酶水解以及对未结合类固醇及其衍生物进行色谱分析后，通过比活性测定确认了各个雌激素和雌激素结合物的身份。