Etches R J
Steroids. 1976 Dec;28(6):763-73. doi: 10.1016/0039-128x(76)90028-3.
A radioimmunoassay for corticosterone was developed using an antibody to corticosterone-21-hemisuccinate:bovine serum albumin. The assay possessed good specificity, sensitivity and reproducibility and required minimal sample preparation. Tests of adrenal function showed that stimulation of the adrenal with exogenous ACTH and with dexamethasone caused an increase and decrease, respectively, in plasma concentrations of corticosterone. Exposure to cold environmental temperatures caused an increase in plasma corticosterone. Handling and the removal of blood samples by venepuncture had no effect upon the concentration of corticosterone. It was concluded that this assay would accurately measure the response to stresses which affect the pituitary-adrenal axis.
利用抗皮质酮 - 21 - 半琥珀酸酯：牛血清白蛋白抗体开发了一种皮质酮放射免疫测定法。该测定法具有良好的特异性、灵敏度和可重复性，且所需样品前处理最少。肾上腺功能测试表明，用外源性促肾上腺皮质激素（ACTH）和地塞米松刺激肾上腺分别导致血浆皮质酮浓度升高和降低。暴露于寒冷环境温度会导致血浆皮质酮升高。处理以及通过静脉穿刺采集血样对皮质酮浓度没有影响。得出的结论是，该测定法将准确测量对影响垂体 - 肾上腺轴的应激的反应。
