Li Yunchao, Li Paul C H, Parameswaran M Ash, Yu Hua-Zhong
Department of Chemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
Anal Chem. 2008 Nov 15;80(22):8814-21. doi: 10.1021/ac801420h. Epub 2008 Oct 24.
In this paper, we report a novel and cost-effective fabrication technique to produce electrode arrays that can be used for monitoring and electrical manipulation of the molecular orientation of DNA self-assembled monolayers (SAMs) on gold. The electrode arrays were prepared from gold coated glass sides or compact discs (CD-Rs) by using standard office inkjet printers without any hardware or software modifications. In this method, electrode arrays of varied shape and size (from submillimeter to centimeter) can be rapidly fabricated and are suitable for standard electrochemical measurements. We were able to use a dual-channel potentiostat to control the electrodes individually and a fluorescence (FL) scanner to image the electrode array simultaneously. With such an integrated modulation setup, the structural switching behavior (from "lying" to "standing" position) and the enhanced hybridization reactivity of thiolate DNA SAMs on gold under potential control have been successfully demonstrated.
在本文中，我们报告了一种新颖且具有成本效益的制造技术，用于生产可用于监测和电操纵金表面DNA自组装单分子层（SAMs）分子取向的电极阵列。电极阵列是通过使用标准办公喷墨打印机在未对任何硬件或软件进行修改的情况下，由涂金的玻璃片或光盘（CD-R）制备而成。用这种方法，可以快速制造出各种形状和尺寸（从亚毫米到厘米）的电极阵列，并且适用于标准电化学测量。我们能够使用双通道恒电位仪分别控制电极，并使用荧光（FL）扫描仪同时对电极阵列进行成像。通过这种集成调制设置，成功证明了在电位控制下金表面硫醇盐DNA SAMs的结构切换行为（从“平躺”到“直立”位置）以及增强的杂交反应活性。
