Auboiron S, Bauchart D, David L
U.R. Métabolismes Energétique et Lipidique, INRA, Centre Clermont-Theix, Saint-Genès Champanelle, France.
J Chromatogr. 1991 Jun 28;547(1-2):411-8. doi: 10.1016/s0021-9673(01)88664-7.
Thin-layer chromatography coupled with flame ionization detection was used to develop a method to separate and to determine simultaneously three polyether carboxylic ionophore antibiotics (abierixin, nigericin and grisorixin) produced by Streptomyces hygroscopicus NRRL B 1865. Various proportions of chloroform, methanol and formic acid (or acetic acid as a substitute for formic acid) were used in the developing solvent to determine changes in RF values of the antibiotics and to allow conditions for maximum resolution to be obtained. Development on Chromarods SII with chloroform-methanol-formic acid (97:4:0.6, v/v/v) gave satisfactory and reliable separations of the three polyether antibiotics. Under these conditions, the internal standard methyl desoxycholate was found to be suitable for their simultaneous determination in the lipid extracts of Streptomyces hygroscopicus NRRL B 1865.
采用薄层色谱法结合火焰离子化检测技术，开发了一种用于分离并同时测定吸水链霉菌NRRL B 1865产生的三种聚醚羧酸离子载体抗生素（阿比里辛、尼日利亚菌素和格利索里辛）的方法。在展开溶剂中使用不同比例的氯仿、甲醇和甲酸（或用乙酸替代甲酸），以确定抗生素的比移值变化，并获得最大分离度的条件。在Chromarods SII上用氯仿 - 甲醇 - 甲酸（97:4:0.6，v/v/v）展开，对这三种聚醚抗生素实现了令人满意且可靠的分离。在这些条件下，发现内标甲基脱氧胆酸盐适用于在吸水链霉菌NRRL B 1865的脂质提取物中同时测定这三种抗生素。
