Wang Zhuo, Hu Ting, Cao Li-Zhi, Kang Rui, Zhao Ming-Yi, Yu Yan, Xu Wang-Qiong
Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2008 Oct;10(5):620-4.
To investigate whether WASP/Verprolin homologous protein 1 (WAVE1) plays a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
WAVE1 mRNA and protein expression in bone marrow mononuclear cells (BMMCs) was measured by RT-PCR and Western blotting respectively in 4 children with ALL relapse, 15 children with ALL in complete remission (CR) and 40 children with newly diagnosed ALL. Ten normal bone marrow samples were used as controls. Jurkat cells were treated with different concentrations of adriamycin (ADM). The cell proliferation was detected with MTT. The apoptosis rate was measured by flow cytometry. WAVE1 mRNA and protein expression of Jurkat cells treated with ADM was detected by RT-PCR and Western blotting respectively.
WAVE1 was not expressed or weakly expressed in BMMCs from normal controls and patients with ALL in CR. Higher WAVE1 mRNA and protein expression was found in BMMCs from patients with newly diagnosed ALL and patients with relapse ALL when compared with the controls and the patients in CR (P<0.01). ADM significantly inhibited the proliferation of the Jurkat cells and the inhibitory effect was dose-and time-dependent (P<0.05). After ADM treatment for 24 hrs, the percentage of apoptosis cells increased significantly and WAVE1 mRNA and protein expression of Jurkat cells decreased significantly when compared with the untreated controls (P<0.05).
The WAVE1 expression increased in children with ALL. WAVE1 may be related to the development of ALL and may be severed as a marker for the evaluation of the severity of ALL in children.
探讨WASP/维普洛林同源蛋白1（WAVE1）在儿童急性淋巴细胞白血病（ALL）发病机制中的作用。
分别采用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测4例ALL复发患儿、15例ALL完全缓解（CR）患儿及40例新诊断ALL患儿骨髓单个核细胞（BMMCs）中WAVE1 mRNA和蛋白表达。选取10例正常骨髓样本作为对照。用不同浓度阿霉素（ADM）处理Jurkat细胞。采用噻唑蓝（MTT）法检测细胞增殖情况。通过流式细胞术检测细胞凋亡率。分别用RT-PCR和蛋白质免疫印迹法检测ADM处理后Jurkat细胞中WAVE1 mRNA和蛋白表达。
正常对照及ALL-CR患儿的BMMCs中WAVE1未表达或低表达。与对照组及ALL-CR患儿相比，新诊断ALL患儿及ALL复发患儿的BMMCs中WAVE1 mRNA和蛋白表达更高（P<0.01）。ADM显著抑制Jurkat细胞增殖，且抑制作用呈剂量和时间依赖性（P<0.05）。ADM处理24小时后，凋亡细胞百分比显著增加，与未处理对照组相比，Jurkat细胞中WAVE1 mRNA和蛋白表达显著降低（P<0.05）。
ALL患儿中WAVE-1表达增加。WAVE1可能与ALL的发生发展有关，可作为评估儿童ALL严重程度的指标。
