Yeh Shinn-Pyng, Chen Ying-Nan, Hsieh Shu-Ling, Cheng Winton, Liu Chun-Hung
Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung 916, Taiwan, ROC.
Fish Shellfish Immunol. 2009 Apr;26(4):582-8. doi: 10.1016/j.fsi.2008.09.010. Epub 2008 Oct 1.
In the present study, we investigated immunological changes in viral-infected white shrimp, Litopenaeus vannamei. White shrimp were infected with white spot syndrome virus (WSSV) or co-infected with WSSV and infectious hypodermal and hematopoietic necrosis virus (IHHNV) as detected by polymerase chain reaction (PCR). The complete (100%) mortality rate of shrimp was caused by viral infection due to immune parameters being suppressed including decreases in phenoloxidase activity, total hemocyte counts, differential hemocyte counts, and the gene expressions of prophenoloxidase and peroxinectin. In addition, increases in lipopolysaccharide and beta-1,3-glucan-binding protein of hemocytes and the hepatopancreas, and respiratory bursts per cell, and a decrease in superoxide dismutase were found in viral-infected shrimp, which may have been related to the defense against viral infection.
在本研究中，我们调查了病毒感染的凡纳滨对虾的免疫变化。通过聚合酶链反应（PCR）检测发现，凡纳滨对虾感染了白斑综合征病毒（WSSV），或同时感染了WSSV和传染性皮下和造血组织坏死病毒（IHHNV）。由于免疫参数受到抑制，包括酚氧化酶活性降低、总血细胞计数减少、不同类型血细胞计数减少以及酚氧化酶原和过氧化物酶基因表达下降，病毒感染导致对虾出现了100%的全死亡率。此外，在病毒感染的对虾中还发现，血细胞和肝胰腺中的脂多糖和β-1,3-葡聚糖结合蛋白增加、每个细胞的呼吸爆发增加以及超氧化物歧化酶减少，这些可能与抵抗病毒感染有关。
