Mitev G, Tekerlekov P, Nikolova E
Vet Med Nauki. 1976;13(7):91-5.
Experiments were carried out to cultivate the foot-and-mouth disease virus under laboratory conditions in a suspension of the BHK-21 cell line. Results showed that the number of cells cultivated in a susfor 48 to 72 hours of incubation reaches up to 2.2 X 10(6). It was also found that certain conditions should be observed for the successful cultivation of the cells in suspension, such as aeration, pH, and respective temperature. It was demonstrated that foot-and-mouth diseases viruses obtained from BHK cells propagating in suspension possess good antigenic and immunogenic properties.
在实验室条件下,利用BHK - 21细胞系的悬浮液开展了口蹄疫病毒培养实验。结果显示,在悬浮液中培养48至72小时的细胞数量可达2.2×10⁶ 。还发现,要成功进行悬浮细胞培养,需注意某些条件,如通气、pH值和相应温度。结果表明,从悬浮培养的BHK细胞中获得的口蹄疫病毒具有良好的抗原性和免疫原性。