Aresha M, Sanath M, Deepika F, Renu W, Anura B, Chanditha H, Wimaladharma A, Rajitha W
Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
Trop Biomed. 2008 Aug;25(2):100-6.
Plasmodim vivax malaria accounts for more than 90% of malaria cases in Sri Lanka. There is limited information on the genetic heterogeneity of P. vivax in endemic areas of the country. Here we have assessed the potential of two P. vivax genes as genetic markers for their use in genotyping parasites collected from the field. DNA extracted from Geimsa-stained P. vivax positive slides were genotyped at two polymorphic loci: the P. vivax merozoite surface protein 3- alpha (Pvmsp-3alpha) and circumsporozoite protein (Pvcs). Analysis of these two genetic markers revealed 11 distinguishable variant types from the two genetic markers: 4 for Pvcs and 7 for Pvmsp-3alpha. The results indicate that the P. vivax parasite population is highly diverse in Sri Lanka, despite the low level of transmission.
间日疟原虫疟疾占斯里兰卡疟疾病例的90%以上。该国流行地区间日疟原虫的遗传异质性信息有限。在此，我们评估了两个间日疟原虫基因作为遗传标记用于对从野外收集的寄生虫进行基因分型的潜力。从吉姆萨染色的间日疟原虫阳性玻片上提取的DNA在两个多态性位点进行基因分型：间日疟原虫裂殖子表面蛋白3-α（Pvmsp-3α）和环子孢子蛋白（Pvcs）。对这两个遗传标记的分析揭示了来自这两个遗传标记的11种可区分的变异类型：Pvcs有4种，Pvmsp-3α有7种。结果表明，尽管传播水平较低，但斯里兰卡的间日疟原虫寄生虫种群高度多样化。
