对斯里兰卡间日疟原虫 merozoite 表面蛋白 3α 基因和两个微卫星位点的多态性分析：斯里兰卡人群结构的证据。
Analysis of polymorphisms in the merozoite surface protein-3α gene and two microsatellite loci in Sri Lankan Plasmodium vivax: evidence of population substructure in Sri Lanka.
机构信息
Centre for Medical Parasitology, Department of International Health, Immunology and Microbiology, Copenhagen University Hospital, Denmark.
出版信息
Am J Trop Med Hyg. 2011 Dec;85(6):994-1001. doi: 10.4269/ajtmh.2011.11-0338.
The geographical distribution of genetic variation in Plasmodium vivax samples (N = 386) from nine districts across Sri Lanka is described using three markers; the P. vivax merozoite surface protein-3α (Pvmsp-3α) gene, and the two microsatellites m1501 and m3502. At Pvmsp-3α, 11 alleles were found with an expected heterozygosity (H(e)) of 0.81, whereas at m1501 and m3502, 24 alleles (H(e) = 0.85) and 8 alleles (H(e) = 0.74) were detected, respectively. Overall, 95 unique three locus genotypes were detected among the 279 samples positive at all three loci (H(e) = 0.95). Calculating the pairwise fixation index (F(ST)) revealed statistically significant population structure. The presence of identical 2-loci microsatellite genotypes in a significant proportion of samples revealed local clusters of closely related isolates contributing to strong linkage disequilibrium between marker alleles. The results show evidence of high genetic diversity and possible population substructure of P. vivax populations in Sri Lanka.
描述了来自斯里兰卡 9 个地区的 386 个间日疟原虫样本（N = 386）中三种标记物的遗传变异的地理分布：间日疟原虫裂殖子表面蛋白-3α（Pvmsp-3α）基因和两个微卫星 m1501 和 m3502。在 Pvmsp-3α 中发现了 11 个等位基因，预期杂合度（H（e））为 0.81，而在 m1501 和 m3502 中分别检测到 24 个等位基因（H（e）= 0.85）和 8 个等位基因（H（e）= 0.74）。在所有三个基因座均呈阳性的 279 个样本中，总共检测到 95 个独特的三基因座基因型（H（e）= 0.95）。计算成对固定指数（F（ST））显示出统计学上显著的种群结构。大量样本中存在相同的 2 个微卫星基因座基因型表明存在密切相关的本地分离群，导致标记等位基因之间存在强烈的连锁不平衡。结果表明，斯里兰卡间日疟原虫种群存在高度遗传多样性和可能的种群亚结构。
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