Gentschev I, Stritzker J, Hofmann E, Weibel S, Yu Y A, Chen N, Zhang Q, Bullerdiek J, Nolte I, Szalay A A
Genelux Corporation, San Diego Science Center, San Diego, CA 92109, USA.
Cancer Gene Ther. 2009 Apr;16(4):320-8. doi: 10.1038/cgt.2008.87. Epub 2008 Oct 24.
Mammary cancers together with cancers of the skin account for about 60% of the total cancers occurring in dogs. The veterinary options for therapeutic management of canine mammary cancer are limited and prognosis for such patients is poor. In this study, we analyzed the functionality of the oncolytic vaccinia virus strain GLV-1h68 as a possible therapeutic agent for canine mammary cancer. Cell culture data demonstrated that GLV-1h68 efficiently infected and destroyed cells of the canine mammary adenoma cell line ZMTH3. Furthermore, after systemic administration this attenuated vaccinia virus strain primarily replicated in canine tumor xenografts in nude mice. The efficient tumor colonization process resulted in inhibition of tumor growth and drastic reduction of tumor size. This is the first report demonstrating that vaccinia virus is an effective tool for the therapy of canine mammary cancers, which might next be applied to dogs with breast tumors.
乳腺癌与皮肤癌一起约占犬类发生的所有癌症的60%。犬类乳腺癌治疗管理的兽医选择有限，此类患者的预后较差。在本研究中，我们分析了溶瘤痘苗病毒株GLV-1h68作为犬类乳腺癌可能治疗剂的功能。细胞培养数据表明，GLV-1h68能有效感染并破坏犬乳腺腺癌细胞系ZMTH3的细胞。此外，全身给药后，这种减毒痘苗病毒株主要在裸鼠的犬肿瘤异种移植中复制。有效的肿瘤定植过程导致肿瘤生长受到抑制，肿瘤大小大幅减小。这是第一份证明痘苗病毒是治疗犬类乳腺癌有效工具的报告，接下来可能会应用于患有乳腺肿瘤的犬类。
