Su Chin-Cheng, Chen Guang-Wei, Lin Jaung-Geng
Buddhist Tzu Chi General Hospital, Hualien, Division of General Surgery, Hualien 970, Taiwan, ROC.
Int J Mol Med. 2008 Nov;22(5):613-8.
Tanshinone I (Tan-I) and tanshinone IIA (Tan-IIA) were isolated from Danshen (Salviae Miltiorrhizae Radix), a widely prescribed traditional herbal medicine that is used to treat cardiovascular and dysmenorrhea diseases. In our previous study, Tan-IIA was demonstrated to induce apoptosis in human colon cancer Colo 205 cells. However, the effect of Tan-I on human colon cancer cells is not clearly understood yet. In this study, the anti-growth and apoptosis-eliciting effects of Tan-I, as well as its cellular mechanisms of actions, were investigated in Colo 205 human colon cancer cells. Tan-I reduced cell growth in a concentration-dependent manner, inducing apoptosis accompanied by an increase in TUNEL staining and in cells in the sub-G1 fraction. The expression of p53, p21, bax and caspase-3 increased in Tan-I-treated cells. In addition, the cell cycle analysis showed G0/G1 arrest. These findings suggest that Tan-I induces apoptosis in Colo 205 cells through both mitochondrial-mediated intrinsic cell-death pathways and p21-mediated G0/G1cell cycle arrest. Accordingly, the therapeutic potential of Tan-I for colon cancer deserves further study.
丹参酮I（Tan-I）和丹参酮IIA（Tan-IIA）是从丹参（Salviae Miltiorrhizae Radix）中分离得到的，丹参是一种广泛应用于治疗心血管疾病和痛经的传统草药。在我们之前的研究中，已证明Tan-IIA可诱导人结肠癌Colo 205细胞凋亡。然而，Tan-I对人结肠癌细胞的作用尚不清楚。在本研究中，我们在Colo 205人结肠癌细胞中研究了Tan-I的抗生长和诱导凋亡作用及其细胞作用机制。Tan-I以浓度依赖的方式降低细胞生长，诱导凋亡，同时TUNEL染色增加，亚G1期细胞增多。Tan-I处理的细胞中p53、p21、bax和caspase-3的表达增加。此外，细胞周期分析显示G0/G1期阻滞。这些发现表明，Tan-I通过线粒体介导的内源性细胞死亡途径和p21介导的G0/G1期细胞周期阻滞诱导Colo 205细胞凋亡。因此，Tan-I对结肠癌的治疗潜力值得进一步研究。
