Wagner Jasenka, Dzijan Snjezana, Marjanović Damir, Lauc Gordan
University of Osijek School of Medicine, DNA Laboratory, J. Huttlera 4, 31000, Osijek, Croatia.
Int J Legal Med. 2009 Jan;123(1):75-9. doi: 10.1007/s00414-008-0292-9. Epub 2008 Oct 24.
Prenatal paternity analysis can be performed only after invasive sampling of chorionic villi or amnionic fluid. Aiming to enable noninvasive paternity testing, we attempted to amplify fetal alleles from maternal plasma. Cell-free DNA was isolated from plasma of 20 pregnant women and amplified with ampFLSTR Identifiler and ampFLSTR Yfiler kits. Unfortunately, autosomal fetal alleles were heavily suppressed by maternal DNA, and the only locus that was reliably amplified with AmpFLSTR Identifiler kit was amelogenin, which revealed only fetal gender. Much better success was obtained with AmpFLSTR Yfiler kit, which, in the case of male fetuses, successfully amplified between six and 16 fetal loci. All amplified fetal alleles matched the alleles of their putative fathers, confirming the tested paternity. To the best of our knowledge, this is a first report of noninvasive prenatal paternity testing.
产前亲子鉴定只能在对绒毛膜绒毛或羊水进行侵入性采样后进行。为了实现无创亲子鉴定，我们尝试从孕妇血浆中扩增胎儿等位基因。从20名孕妇的血浆中分离出游离DNA，并用ampFLSTR Identifiler和ampFLSTR Yfiler试剂盒进行扩增。不幸的是，常染色体胎儿等位基因受到母体DNA的严重抑制，使用AmpFLSTR Identifiler试剂盒唯一能可靠扩增的位点是牙釉蛋白，它仅显示胎儿性别。使用AmpFLSTR Yfiler试剂盒获得了更好的结果，在男性胎儿的情况下，成功扩增了6至16个胎儿位点。所有扩增的胎儿等位基因都与其推定父亲的等位基因匹配，证实了检测的亲子关系。据我们所知，这是无创产前亲子鉴定的首次报告。
