Gornik Ivan, Marcikic Mladen, Kubat Milovan, Primorac Dragan, Lauc Gordan
University of Osijek School of Medicine, DNA Laboratory, J. Huttlera 4, 31000 Osijek, Croatia.
Int J Legal Med. 2002 Oct;116(5):255-7. doi: 10.1007/s00414-001-0280-9. Epub 2002 Jun 12.
Since February 2001 the process of DNA identification of war victims in Croatia relies on the database of over 3,000 9-locus (D3S1358, vWA, FGA, TH01, TPOX, CSF1PO, D5S818, D13S317 and D7S820) STR genotypes of relatives of missing persons. Instead of a targeted approach to DNA typing, the genotype of each skeletal remains analysed is compared to all genotypes in the database to identify potential parents and children. Although this approach has significantly increased the pace of identification by DNA typing, non-targeted matching in a database containing several thousand genotypes considerably decreases the significance of inclusion, especially when identification is based on reverse paternity analysis. To support this statistical prediction we present 3 cases of 10 STR loci matches and 1 case of 11 STR loci matches between a child, child's mother and skeletal remains that did not originate from a father of that child.
自2001年2月以来，克罗地亚战争受害者的DNA鉴定工作依赖于一个数据库，该数据库包含3000多个失踪人员亲属的9个基因座（D3S1358、vWA、FGA、TH01、TPOX、CSF1PO、D5S818、D13S317和D7S820）的STR基因型。与有针对性的DNA分型方法不同，对每具分析的骨骼遗骸的基因型与数据库中的所有基因型进行比较，以识别潜在的父母和子女。尽管这种方法显著加快了DNA分型的鉴定速度，但在一个包含数千个基因型的数据库中进行非针对性匹配会大大降低包含的显著性，尤其是当鉴定基于反向父系分析时。为支持这一统计预测，我们展示了3例儿童、儿童母亲与并非该儿童父亲的骨骼遗骸之间10个STR基因座匹配的案例以及1例11个STR基因座匹配的案例。
