Edahiro Jun-Ichi, Sumaru Kimio, Ooshima Yuki, Kanamori Toshiyuki
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Biotechnol Bioeng. 2009 Mar 1;102(4):1278-82. doi: 10.1002/bit.22124.
Taking advantage of the phenomenon that animal cells adhering to a culture substrate are temporarily immobilized by light irradiation, we established a technique to manipulate the cells adhering to a culture substrate under microscopic observation. Using this technique, we demonstrated a separation of cells adhering to a culture substrate and fabrication of an elaborately patterned co-culture system.
利用动物细胞附着于培养底物时经光照会暂时固定的现象，我们建立了一种在显微镜观察下操控附着于培养底物的细胞的技术。运用该技术，我们展示了附着于培养底物的细胞的分离以及精心设计的共培养系统的构建。
