Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Biotechnol Bioeng. 2013 Jan;110(1):348-52. doi: 10.1002/bit.24617. Epub 2012 Aug 22.
As a powerful tool of cell screening and cell purification, we developed a novel method to kill adherent cells as cultured on a substrate by micro-projection of incoherent visible light. To kill the cells by the mild light irradiated by electrically controllable micro-projection systems currently available, we introduced the assist of the photo-responsive culture substrates functionalized with a photo-acid-generating polymer. In clear contrast to the existing laser-based methods requiring point scanning, areal micro-projection of blue light with the wavelength 436 nm killed many CHO-K1 cells at a time in the irradiated area on the substrate. The effect of the photo-generated acid was so confined that selective killing of targeted cells was achieved without critical damage to the neighboring cells. Further, we demonstrated the photo-selective killing of the adherent cells after preliminarily patterning through the photo-induced removal of cell adhesion-inhibiting polymer.
作为细胞筛选和细胞纯化的有力工具，我们开发了一种新方法，通过非相干可见光的微喷射来杀死在基质上培养的贴壁细胞。为了通过目前可用的电控微喷射系统温和的光照射杀死细胞，我们引入了对功能化有光产酸聚合物的光响应培养基质的辅助。与现有的基于激光的需要点扫描的方法形成鲜明对比的是，波长为 436nm 的蓝光的大面积微喷射可以一次性杀死基质上照射区域中的许多 CHO-K1 细胞。光生成酸的效果非常局限，因此可以实现对靶细胞的选择性杀伤，而不会对相邻细胞造成严重损伤。此外，我们通过光诱导去除细胞黏附抑制聚合物初步进行图案化后，证明了对贴壁细胞的光选择性杀伤。
