Khatibi Ali, Haghparast Abbas, Shams Jamal, Dianati Elham, Komaki Alireza, Kamalinejad Mohammad
Neuroscience Research Center, Shahid Beheshti University, M.C., P.O. Box 19615-1178, Tehran, Iran.
Neurosci Lett. 2008 Dec 19;448(1):94-8. doi: 10.1016/j.neulet.2008.10.042. Epub 2008 Oct 17.
Rewarding properties of opioids are now accepted and widely discussed. These properties can lead to long-term usage of these substances. The main purpose of this study was to investigate the effects of Cuminum cyminum fruit essential oil (FEO) on the acquisition and expression of morphine-induced conditioned place preference (CPP) in mice. CPP was induced by subcutaneous (s.c.) injection of morphine (5mg/kg) in 3 days conditioning schedule. Intraperitoneal (i.p.) administration of Cumin FEO (0.001%, 0.01%, 0.1%, 0.5%, 1% and 2%; 5 ml/kg) or Tween-80 (0.5%; 5 ml/kg) did not show any conditioning effects. Administration of Cumin FEO (0.001-2%; 5 ml/kg; i.p.), 60 min before test on day 5 (expression) decreased the conditioning scores at the doses of 1% and 2% while i.p. injection of Cumin FEO (0.001-2%; 5 ml/kg), 60 min before morphine injection (5mg/kg; s.c.) during 3 days of conditioning session (acquisition) significantly resulted in decrement of rewarding properties of morphine at the doses of 0.1%, 0.5%, 1% and 2% in dose-dependent manner. Tween-80 as a vehicle did not suppress the acquisition and expression of morphine-induced CPP. The results showed that the C. cyminum fruit essential oil reduces the acquisition and expression of morphine-induced conditioned place preference in mice.
阿片类药物的奖赏特性现已得到认可并被广泛讨论。这些特性可能导致这些物质的长期使用。本研究的主要目的是调查孜然果实精油（FEO）对小鼠吗啡诱导的条件性位置偏爱（CPP）的获得和表达的影响。在3天的条件化程序中，通过皮下（s.c.）注射吗啡（5mg/kg）诱导CPP。腹腔内（i.p.）给予孜然FEO（0.001%、0.01%、0.1%、0.5%、1%和2%；5 ml/kg）或吐温-80（0.5%；5 ml/kg）未显示出任何条件化作用。在第5天测试（表达）前60分钟腹腔内给予孜然FEO（0.001 - 2%；5 ml/kg），剂量为1%和2%时可降低条件化得分，而在3天的条件化过程（获得）中，在吗啡注射（5mg/kg；s.c.）前60分钟腹腔内注射孜然FEO（0.001 - 2%；5 ml/kg），剂量为0.1%、0.5%、1%和2%时，以剂量依赖方式显著降低吗啡的奖赏特性。作为溶剂的吐温-80并未抑制吗啡诱导的CPP的获得和表达。结果表明，孜然果实精油可降低小鼠吗啡诱导的条件性位置偏爱的获得和表达。
