Sönmez Mustafa, Bozkurt Tanzer, Türk Gaffari, Gür Seyfettin, Kizil Meltem, Yüce Abdurrauf
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Firat University, 23119 Elaziğ, Turkey.
Anim Reprod Sci. 2009 Aug;114(1-3):183-92. doi: 10.1016/j.anireprosci.2008.09.007. Epub 2008 Sep 12.
The objectives of this study were to investigate whether the use of intravaginal sponge for estrous synchronization of goats causes oxidative stress, and to examine the effect of administering vitamin E during preovulatory period on reproductive performance of estrous synchronized goats. Estrus was synchronized in 36 non-lactating adult does using intravaginal sponges containing 30 mg of fluorogestane acetate (FGA) for 14 days. All females received 500 IU of eCG at the sponge withdrawal. The goats were allocated at random to two groups balanced for breed, age and body weight. Treatment group (n=18) received 200mg of vitamin E i.m. at the time of sponge removal and again at the time of second artificial insemination. The other 18 goats (control) were administered 1 ml of physiological saline instead of vitamin E on each of these two occasions. All does in estrus was intracervically inseminated at 12 and 24h after the onset of estrus. Blood samples were collected every 72h during the experimental period for evaluation of malondialdehyde (MDA) and vitamin E concentrations. Serum MDA level increased and vitamin E concentration decreased during the period of vaginal sponge application. Following the sponge removal, MDA level declined rapidly to below basal level in the treatment group but remained high in the control group. Conversely, vitamin E concentration increased in the treatment group after the sponge withdrawal and remained at a low level in the control group. No statistically significant differences (P>0.05) were observed between groups in terms of estrous response, conception rate, gestation length or kidding rate. However, the number of multiple births (70.0% versus 50.0%) and prolificacy rate (2.40+/-0.37 versus 1.63+/-0.26 kids per kidding) were significantly higher (P<0.05) for the treatment group than those of the control group. The results indicate that the use of intravaginal sponges for estrous synchronization of goats causes an increase in level of oxidative stress. However, the vitamin E treatment during preovulatory period can prevent the overproduction of reactive oxygen species (ROS), and it may improve the multiple birth rates and the number of kids born in estrous synchronized goats.
本研究的目的是调查使用阴道海绵进行山羊发情同步化是否会引起氧化应激，并研究在排卵前期给予维生素E对发情同步化山羊繁殖性能的影响。使用含30mg醋酸氟孕酮（FGA）的阴道海绵对36只非泌乳成年母羊进行14天的发情同步化处理。所有母羊在取出海绵时注射500IU的孕马血清促性腺激素（eCG）。山羊按品种、年龄和体重随机分为两组。处理组（n = 18）在取出海绵时及第二次人工授精时肌肉注射200mg维生素E。另外18只山羊（对照组）在这两个时间点分别注射1ml生理盐水而非维生素E。所有发情母羊在发情开始后12小时和24小时进行子宫颈内授精。在实验期间每72小时采集血样，以评估丙二醛（MDA）和维生素E浓度。在阴道海绵放置期间，血清MDA水平升高而维生素E浓度降低。取出海绵后，处理组的MDA水平迅速降至基础水平以下，而对照组仍保持较高水平。相反，处理组在取出海绵后维生素E浓度升高，而对照组则维持在较低水平。在发情反应、受孕率、妊娠期长度或产羔率方面，两组之间未观察到统计学显著差异（P>0.05）。然而，处理组的多胎率（70.0%对50.0%）和产仔率（每胎产仔数为2.40±0.37对1.63±0.26只）显著高于（P<0.05）对照组。结果表明，使用阴道海绵进行山羊发情同步化会导致氧化应激水平升高。然而，排卵前期的维生素E处理可防止活性氧（ROS）的过度产生，并且可能提高发情同步化山羊的多胎率和产仔数。
