Chen Han-Qing, Jin Zheng-Yu, Wang Xi-Jin, Xu Xue-Ming, Deng Li, Zhao Jian-Wei
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
Neurosci Lett. 2008 Dec 26;448(2):175-9. doi: 10.1016/j.neulet.2008.10.046. Epub 2008 Oct 19.
Parkinson's disease is a neurodegenerative disorder characterized by progressive degeneration of dopaminergic neurons in the substantia nigra. Accumulating evidence has suggested that inflammation in the brain participates in the pathogenesis of Parkinson's disease. Luteolin, a polyphenolic compound found in foods of plant origin, belongs to the flavone subclass of flavonoids, and has been shown to possess antimutagenic, antitumorigenic, antioxidant and antiinflammatory properties. In this study, we found that luteolin concentration-dependently attenuated the lipopolysaccharide (LPS)-induced decrease in [(3)H]dopamine uptake and loss of tyrosine hydroxylase-immunoreactive neurons in primary mesencephalic neuron-glia cultures. Moreover, luteolin also significantly inhibited LPS-induced activation of microglia and excessive production of tumor necrosis factor-alpha, nitric oxide and superoxide in mesencephalic neuron-glia cultures and microglia-enriched cultures. Our results demonstrate that luteolin may protect dopaminergic neurons from LPS-induced injury and its efficiency in inhibiting microglia activation may underlie the mechanism.
帕金森病是一种神经退行性疾病，其特征是黑质中多巴胺能神经元进行性退化。越来越多的证据表明，脑部炎症参与帕金森病的发病机制。木犀草素是一种存在于植物性食物中的多酚类化合物，属于黄酮类的黄酮子类，已被证明具有抗诱变、抗肿瘤、抗氧化和抗炎特性。在本研究中，我们发现木犀草素浓度依赖性地减弱了脂多糖（LPS）诱导的原代中脑神经元-胶质细胞培养物中[³H]多巴胺摄取的减少以及酪氨酸羟化酶免疫反应性神经元的丧失。此外，木犀草素还显著抑制了LPS诱导的中脑神经元-胶质细胞培养物和富含小胶质细胞的培养物中小胶质细胞的激活以及肿瘤坏死因子-α、一氧化氮和超氧化物的过量产生。我们的结果表明，木犀草素可能保护多巴胺能神经元免受LPS诱导的损伤，其抑制小胶质细胞激活的功效可能是其作用机制。
