Cairns J M, Dunning M J, Ritchie M E, Russell R, Lynch A G
Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB20RE, UK.
Bioinformatics. 2008 Dec 15;24(24):2921-2. doi: 10.1093/bioinformatics/btn557. Epub 2008 Oct 25.
With their many replicates and their random layouts, Illumina BeadArrays provide greater scope fordetecting spatial artefacts than do other microarray technologies. They are also robust to artefact exclusion, yet there is a lack of tools that can perform these tasks for Illumina. We present BASH, a tool for this purpose. BASH adopts the concepts of Harshlight, but implements them in a manner that utilizes the unique characteristics of the Illumina technology. Using bead-level data, spatial artefacts of various kinds can thus be identified and excluded from further analyses.
The beadarray Bioconductor package (version 1.10 onwards), www.bioconductor.org
Illumina BeadArrays具有多个重复样本和随机布局，与其他微阵列技术相比，在检测空间伪影方面具有更大的范围。它们对伪影排除也具有鲁棒性，但缺乏能够为Illumina执行这些任务的工具。我们提出了BASH，一个用于此目的的工具。BASH采用了Harshlight的概念，但以利用Illumina技术独特特性的方式来实现它们。因此，使用珠子水平的数据，可以识别各种空间伪影并将其从进一步分析中排除。
beadarray Bioconductor包（从1.10版起），www.bioconductor.org
