Determination of trenbolone residual in bovine liver by liquid chromatography-mass spectrometry.

A sensitive method was developed and validated for the quantitation of trenbolone residues in bovine liver. Target analytes were extracted from liver homogenate using solid phase cartridges, separated on a Phenyl column and detected using an electrospray ionization mass spectrometer operating in positive ion mode. The mean recovery of the analytes was between 62% and 69%. The method provided detection capabilities of 1 microg/kg for trenbolone. The method is suitable for application in veterinary drug residue in surveillance programme.