在幼鱼发育早期暴露于17β-雌二醇和睾酮的赤睛鱼（Leuciscus cephalus L.）中的性别分化、卵黄蛋白原和11-酮睾酮水平
Sex differentiation and vitellogenin and 11-ketotestosterone levels in chub, Leuciscus cephalus L., exposed to 17 beta-estradiol and testosterone during early development.
作者信息
Zlábek Vladimír, Randák T, Kolárová J, Svobodová Z, Kroupová H
机构信息
Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceské Budejovice, Vodnany, Czech Republic.
出版信息
Bull Environ Contam Toxicol. 2009 Mar;82(3):280-4. doi: 10.1007/s00128-008-9579-2. Epub 2008 Oct 25.
The effects of 17 beta-estradiol (E(2)) and testosterone (T) singly and in combination were tested on juvenile chub (Leuciscus cephalus L.). Vitellogenin (VTG) and 11-ketotestosterone (11-KT) were determined by ELISA in whole body homogenates and the gonads were examined histologically. Testosterone and estradiol, in combination, significantly increased whole body VTG (p < 0.01), but not 11-KT, compared to controls and the T treated groups. The only intersex observed (1/80) was in the combined treatment group. We suggest that VTG measured in whole body homogenates could be used to determine the effects of exogenous steroids in juvenile chub.
分别单独以及联合测试了17β-雌二醇（E₂）和睾酮（T）对幼年期银鲫（Leuciscus cephalus L.）的影响。通过酶联免疫吸附测定法（ELISA）测定了鱼体匀浆中的卵黄蛋白原（VTG）和11-酮睾酮（11-KT），并对性腺进行了组织学检查。与对照组和睾酮处理组相比，睾酮和雌二醇联合使用显著增加了鱼体整体的卵黄蛋白原水平（p < 0.01），但对11-KT水平无显著影响。观察到的唯一一例雌雄同体现象（1/80）出现在联合处理组中。我们认为，测定鱼体匀浆中的卵黄蛋白原可用于确定外源类固醇对幼年期银鲫的影响。
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