Poloni A, Pessi I S, Frazzon A P G, Van Der Sand S T
Departamento de Microbiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Curr Microbiol. 2009 Feb;58(2):153-8. doi: 10.1007/s00284-008-9292-8. Epub 2008 Oct 25.
Bipolaris sorokiniana is a phytopathogenic fungus that causes diseases of cereal crops, such as leaf-spot disease, common root rot, and black point of grain. Because of its great morphological, physiological, and genetic variability, this fungus is difficult to control. The aim of this investigation was to study the variability of isolates of B. sorokiniana by means of vegetative incompatibility. Thirty-five isolates of B. sorokiniana from different geographical regions in Brazil and other countries were used. The vegetative incompatibility between the isolates and the influences of different culture media on these reactions were evaluated. The total protein profile of the isolates was analyzed when the isolates were cultured separately, and in cultures of compatibility and incompatibility reactions. Eighteen of 31 confrontations showed vegetative incompatibility. The results obtained with different culture media for the vegetative compatibility/incompatibility genotypes suggested that the type of substratum influences these reactions. No differences in protein profiles among the isolates were observed. This result suggests that there is no induction of expression of different proteins in vegetative incompatibility reactions.
玉米大斑病菌是一种植物病原真菌，可引发谷类作物病害，如叶斑病、普通根腐病和籽粒黑胚病。由于其在形态、生理和遗传方面具有很大的变异性，这种真菌很难防治。本研究的目的是通过营养体不亲和性来研究玉米大斑病菌分离株的变异性。使用了来自巴西和其他国家不同地理区域的35株玉米大斑病菌分离株。评估了分离株之间的营养体不亲和性以及不同培养基对这些反应的影响。当分离株单独培养以及在亲和性和不亲和性反应培养物中培养时，分析了分离株的总蛋白谱。31次对峙中有18次表现出营养体不亲和性。不同培养基对营养体亲和性/不亲和性基因型的研究结果表明，培养基类型会影响这些反应。未观察到分离株之间蛋白质谱的差异。这一结果表明，在营养体不亲和反应中没有诱导不同蛋白质的表达。
