Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India.
Mycologia. 2002 Nov-Dec;94(6):1051-8.
Variability among 26 isolates of Sclerotium rolfsii collected from various hosts/soil samples and localities in India is reported. The isolates varied in colony morphology, mycelial growth rate, sclerotium formation, teleomorph production and sclerotial size and color. Out of 26 isolates, only 4 produced the teleomorph stage on Cyperus rotundus rhizome meal agar medium. Mycelial incompatibility among the isolates was also seen, and out of 325 combinations, only 29 combinations (8.9%) showed compatible reactions. Based on mycelial compatibility, 13 vegetative incompatibility groups (VCG) were identified among the isolates. HPLC analysis of the ethyl acetate fraction of culture filtrates of the isolates revealed 10-22 peaks. Six peaks were identified as gallic, oxalic, ferulic, indole-3-acetic acid (IAA), chlorogenic, and cinnamic acids. Oxalic, IAA, and cinnamic acids were present in the culture filtrates of all the isolates in varying amounts. The other three phenolic acids were not detected in some of the isolates. A comparative HPLC analysis of sclerotial exudate, sclerotia, mycelia, and culture filtrates of two S. rolfsii isolates (leaf spot- and collar rot-causing) producing different symptoms on their respective hosts revealed variation in the content of phenolic acids, IAA, and oxalic acid.
本文报道了从印度不同宿主/土壤样本和地区收集的 26 株匐柄核瑚菌分离株的变异性。这些分离株在菌落形态、菌丝生长速度、菌核形成、有性型产生和菌核大小及颜色上存在差异。在 26 个分离株中，只有 4 个在香蒲根茎粉琼脂培养基上产生了有性型阶段。还观察到了分离株之间的菌丝不亲和性，在 325 个组合中，只有 29 个组合（8.9%）表现出亲和反应。基于菌丝亲和性，从分离株中鉴定出 13 个营养体不亲和群（VCG）。对分离株培养滤液的乙酸乙酯部分进行 HPLC 分析显示出 10-22 个峰。其中 6 个峰被鉴定为没食子酸、草酸、阿魏酸、吲哚-3-乙酸（IAA）、绿原酸和肉桂酸。所有分离株的培养滤液中均存在草酸、IAA 和肉桂酸，但其含量不同。其他三种酚酸在一些分离株中未被检测到。对两个引起叶斑病和茎基腐病的 S. rolfsii 分离株（在各自的宿主上产生不同症状）的菌核渗出物、菌核、菌丝和培养滤液进行比较 HPLC 分析，发现酚酸、IAA 和草酸的含量存在差异。
