Xie Zheng-De, Wang Lin, Lu Di, Zhou Chun-Ju, Shen Kun-Ling
Laboratory of Virology, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
Zhonghua Zhong Liu Za Zhi. 2008 May;30(5):365-7.
To investigate the correlation of Epstein-Barr virus (EBV) infection and childhood lymphoma.
Paraffin-embedded specimens of lymphoma collected between 1996 and 2005, including 36 Hodgkin lymphomas (HL) and 51 non-Hodgkin lymphomas (NHL), were included in this study. Paraffin-embedded specimens of reactive hyperplasia of lymph nodes (RL) collected during the same period were used as controls. Immunohistochemical (IHC) assay was used to detect EBV-LMP1 and in situ hybridization (ISH) to detect EBV-EBERs.
EBV was detected in 72.2% (26/36) of the Hodgkin lymphomas, 15.7% (8/51) of the non-Hodgkin lymphomas and 33.3% (15/45) of the reactive hyperplasia of lymph nodes. There was a significant difference among Hodgkin lymphoma, non-Hodgkin lymphoma and RL (P = 0. 000).
Childhood Hodgkin lymphoma is closely correlated with Epstein-Barr virus infection. However, the low rate of EBV infection detected in childhood non-Hodgkin lymphoma might be due to heterogeneous distribution of pathological types in this study.
探讨爱泼斯坦-巴尔病毒（EBV）感染与儿童淋巴瘤的相关性。
本研究纳入1996年至2005年间收集的淋巴瘤石蜡包埋标本，包括36例霍奇金淋巴瘤（HL）和51例非霍奇金淋巴瘤（NHL）。同期收集的淋巴结反应性增生（RL）石蜡包埋标本用作对照。采用免疫组织化学（IHC）检测EBV-LMP1，原位杂交（ISH）检测EBV-EBERs。
霍奇金淋巴瘤中72.2%（26/36）检测到EBV，非霍奇金淋巴瘤中15.7%（8/51）检测到EBV，淋巴结反应性增生中33.3%（15/45）检测到EBV。霍奇金淋巴瘤、非霍奇金淋巴瘤和RL之间存在显著差异（P = 0.000）。
儿童霍奇金淋巴瘤与爱泼斯坦-巴尔病毒感染密切相关。然而，本研究中儿童非霍奇金淋巴瘤EBV感染率较低可能是由于病理类型分布不均所致。
