Teramoto N, Sarker A B, Tonoyama Y, Yoshino T, Hayashi K, Takahashi K, Akagi T
Department of Pathology, Okayama University Medical School, Japan.
Cancer. 1996 Jun 1;77(11):2339-47. doi: 10.1002/(SICI)1097-0142(19960601)77:11<2339::AID-CNCR24>3.0.CO;2-X.
The Epstein-Barr virus (EBV) has been frequently detected in lymphoid malignancies. However, EBV infection in the nonneoplastic cells of lymphoid malignancies has not been extensively studied.
Four hundred nine cases of lymphoid malignancies including 377 non-Hodgkin's lymphoma (NHL) and 32 Hodgkin's disease (HD) were examined for EBV infection by EBER-1 in situ hybridization (EBER-ISH), immunostaining against LMP-1, Epstein-Barr nuclear antigen 2 (EBNA2) and ZEBRA, and Southern hybridization using a BamHIW fragment as a probe. Double staining with EBER-ISH and immunostaining against CD20, CD45RO, and LMP-1 was performed in selected cases.
Although EBER-1-positive cells (EPCs) were detected in 49 of 276 B-cell lymphomas, 31 of 100 T-cell lymphomas, 1 of 1 natural killer-cell lymphoma, and 17 of 32 HDs, almost all of the tumor cells were exclusively EBER-1-positive in the 10 NHL cases. Some EPCs were of different cell lineages than the tumor cells in 15 of the 26 NHLs examined by double staining. LMP-1, EBNA2, and ZEBRA were detected in 22, 4, and 3 cases, respectively. In 4 LMP-1-positive HDs, double staining revealed that some EBER-1-positive Reed-Sternberg cells were negative for LMP-1, EBV genomic DNA was detected in 8 of the 39 examined cases.
T-cell lymphomas contained EPCs more frequently than B-cell lymphomas. Nonneoplastic lymphocytes were infected with EBV more frequently than lymphoma cells. Rowe's latency II may be unstable in lymphoid malignancies. Some NHLs, especially T-cell lymphoma, may provide favorable conditions for EBV infection of nonneoplastic lymphocytes.
爱泼斯坦-巴尔病毒（EBV）在淋巴系统恶性肿瘤中经常被检测到。然而，淋巴系统恶性肿瘤中非肿瘤细胞的EBV感染尚未得到广泛研究。
通过EBER-1原位杂交（EBER-ISH）、针对LMP-1、爱泼斯坦-巴尔核抗原2（EBNA2）和ZEBRA的免疫染色以及使用BamHIW片段作为探针的Southern杂交，对409例淋巴系统恶性肿瘤进行了EBV感染检测，其中包括377例非霍奇金淋巴瘤（NHL）和32例霍奇金病（HD）。在选定病例中进行了EBER-ISH与针对CD20、CD45RO和LMP-1的免疫染色的双重染色。
虽然在276例B细胞淋巴瘤中的49例、100例T细胞淋巴瘤中的31例、1例自然杀伤细胞淋巴瘤和32例HD中的17例中检测到EBER-1阳性细胞（EPC），但在10例NHL病例中，几乎所有肿瘤细胞均为EBER-1阳性。在通过双重染色检查的26例NHL中的15例中，一些EPC与肿瘤细胞的细胞谱系不同。分别在22例、4例和3例中检测到LMP-1、EBNA2和ZEBRA。在4例LMP-1阳性的HD中，双重染色显示一些EBER-1阳性的里德-斯腾伯格细胞LMP-阴性，在39例检查病例中的8例中检测到EBV基因组DNA。
T细胞淋巴瘤比B细胞淋巴瘤更频繁地含有EPC。非肿瘤性淋巴细胞比淋巴瘤细胞更频繁地感染EBV。Rowe潜伏期II在淋巴系统恶性肿瘤中可能不稳定。一些NHL，尤其是T细胞淋巴瘤，可能为非肿瘤性淋巴细胞的EBV感染提供有利条件。
