Shi Rui-ru, Zhang Jian-yuan, Yuan Xue-qin, Sun Zhao-gang, Li Chuan-you
Central Laboratory of Henan Provincial Chest Hospital, Zhengzhou 450003, China.
Zhonghua Yi Xue Za Zhi. 2008 May 27;88(20):1376-9.
To determine the rpsL and rrs gene mutation in Mycobacterium tuberculosis (M. tuberculosis) and compare the consistency between the results of denaturing high-performance liquid chromatography (DHPLC) and those of DNA sequencing.
The values of streptomycin minimum inhibitory concentration (MIC) against 215 M. tuberculosis clinical isolates, 115 being streptomycin-resistant and 100 being susceptible by a routine proportional method, were tested by DHPLC. DNA sequencing was conducted to detect the rpsL and rrs mutation.
98 of the 115 streptomycin-resistant isolates (85.2%) harbored rpsL and/or rrs mutation, 76.5% of which being rpsL mutation (88/115). There was no significant correlation between the MIC values and mutation types. No mutation was found in all the susceptible isolates. There was a complete consistency between the DHPLC results and those of DNA sequencing.
DHPLC can be regarded as a useful and powerful tool to detect the streptomycin resistance detection in M. tuberculosis.
确定结核分枝杆菌（M. tuberculosis）中rpsL和rrs基因突变情况，并比较变性高效液相色谱法（DHPLC）与DNA测序结果之间的一致性。
采用DHPLC检测215株结核分枝杆菌临床分离株的链霉素最低抑菌浓度（MIC）值，其中115株对链霉素耐药，100株对链霉素敏感，通过常规比例法进行检测。进行DNA测序以检测rpsL和rrs基因突变。
115株链霉素耐药分离株中有98株（85.2%）存在rpsL和/或rrs基因突变，其中76.5%为rpsL基因突变（88/115）。MIC值与突变类型之间无显著相关性。所有敏感分离株均未发现突变。DHPLC结果与DNA测序结果完全一致。
DHPLC可被视为检测结核分枝杆菌链霉素耐药性的一种有用且强大的工具。
