Joseph James A, Fisher Derek R, Cheng Vivian, Rimando Agnes M, Shukitt-Hale Barbara
US Department of Agriculture, Agricultural Research Service, Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts 02111, USA.
J Agric Food Chem. 2008 Nov 26;56(22):10544-51. doi: 10.1021/jf802279h.
Research suggests that polyphenolic compounds contained in fruits and vegetables that are rich in color may have potent antioxidant and anti-inflammatory activities. The present studies determined if stilbene (e.g., resveratrol) compounds would be efficacious in reversing the deleterious effects of aging in 19 month old Fischer 344 rats. Experiment I utilized resveratrol and six resveratrol analogues and examined their efficacies in preventing dopamine-induced decrements in calcium clearance following oxotremorine-induced depolarization in COS-7 cells transfected with M1 muscarinic receptors (MAChR) that we have shown previously to be sensitive to oxidative stressors. Experiment II utilized the most efficacious analogue (pterostilbene) from experiment I and fed aged rats a diet with a low (0.004%) or a high (0.016%) concentration of pterostilbene. Results indicated that pterostilbene was effective in reversing cognitive behavioral deficits, as well as dopamine release, and working memory was correlated with pterostilbene levels in the hippocampus.
研究表明，富含色素的水果和蔬菜中所含的多酚类化合物可能具有强大的抗氧化和抗炎活性。目前的研究确定了芪类化合物（如白藜芦醇）是否能有效逆转19月龄Fischer 344大鼠衰老的有害影响。实验I使用了白藜芦醇和六种白藜芦醇类似物，并检测了它们在转染了M1毒蕈碱受体（MAChR）的COS-7细胞中，预防氧化震颤素诱导去极化后多巴胺引起的钙清除率下降的效果，我们之前已证明该细胞对氧化应激源敏感。实验II使用了实验I中最有效的类似物（紫檀芪），给老年大鼠喂食低浓度（0.004%）或高浓度（0.016%）紫檀芪的饮食。结果表明，紫檀芪能有效逆转认知行为缺陷以及多巴胺释放，且工作记忆与海马体中紫檀芪水平相关。
