Martín Maria Cruz, del Rio Beatriz, Martínez Noelia, Magadán Alfonso H, Alvarez Miguel A
Instituto de Productos Lácteos de Asturias (CSIC), Apdo. de Correos 85, 33300 Villaviciosa, Asturias, Spain.
Food Microbiol. 2008 Dec;25(8):978-82. doi: 10.1016/j.fm.2008.07.004. Epub 2008 Jul 11.
One of the main microbiological problems of the dairy industry is the susceptibility of starter bacteria to virus infections. Lactobacillus delbrueckii, a component of thermophilic starter cultures used in the manufacture of several fermented dairy products, including yogurt, is also sensitive to bacteriophage attacks. To avoid the problems associated with these viruses, quick and sensitive detection methods are necessary. In the present study, a fast real-time quantitative polymerase chain reaction assay for the direct detection and quantification of L. delbrueckii phages in milk was developed. A set of primers and a TaqMan MGB probe was designed, based on the lysin gene sequence of different L. delbrueckii phages. The results show the proposed method to be a rapid (total processing time 30 min), specific and highly sensitive technique for detecting L. delbrueckii phages in milk.
乳制品行业的主要微生物问题之一是发酵剂细菌易受病毒感染。德氏乳杆菌是用于生产多种发酵乳制品（包括酸奶）的嗜热发酵剂培养物的一个组成部分，它也对噬菌体攻击敏感。为避免与这些病毒相关的问题，快速且灵敏的检测方法是必要的。在本研究中，开发了一种用于直接检测和定量牛奶中德氏乳杆菌噬菌体的快速实时定量聚合酶链反应检测方法。基于不同德氏乳杆菌噬菌体的溶菌酶基因序列设计了一组引物和一个TaqMan MGB探针。结果表明，所提出的方法是一种快速（总处理时间30分钟）、特异且高度灵敏的检测牛奶中德氏乳杆菌噬菌体的技术。
