Hirose T
Acta Endocrinol (Copenh). 1977 Feb;84(2):349-56. doi: 10.1530/acta.0.0840349.
Isolated adrenal cells of rabbits aged from 0 h to day 40 after birth were incubated for 2 h in the presence of ACTH. Thin-layer chromatography and the fluorimetric method were used to evaluate the products of these adrenal cells separately for cortisol and corticosterone. Cortisol production by adrenal cells in response to ACTH (0.1 mU) was found to be highest between 0 and 8 h after birth (0.522 +/- 0.049 mug/105 cells; mean +/- SEM). A decrease was observed at 9-20 h and this was further followed by a steady decrease up to day 35-40. Corticosterone production was 0.206 +/- 0.042 mug/105 cells between 0 and 8 h after birth. Subsequently, this level was evenly maintained up to 12-14, but it tended to increase on day 35-40. The level of cortisol production was significantly higher than that of corticosterone production at 0-20 h after birth, while the former was significantly lower than the latter from day 12-14 onwards. The ratio of corticosterone to total corticosteroids (cortisol + corticosterone) was very low (0.28 +/- 0.04) at 0-8 h after birth, but showed a progressive rise up to day 35-40, when it was 0.95 +/- 0.01. The results of the present study show that cortisol is the pre-dominant product of the adrenal cells in rabbits during early post-natal life.
将出生后0小时至40天的兔分离肾上腺细胞在促肾上腺皮质激素（ACTH）存在的情况下孵育2小时。采用薄层色谱法和荧光法分别评估这些肾上腺细胞产生的皮质醇和皮质酮。发现肾上腺细胞对促肾上腺皮质激素（0.1 mU）产生的皮质醇在出生后0至8小时最高（0.522±0.049 μg/105个细胞；平均值±标准误）。在9至20小时观察到下降，随后直至第35 - 40天持续稳定下降。出生后0至8小时皮质酮产量为0.206±0.042 μg/105个细胞。随后，该水平在12 - 14小时前保持稳定，但在第35 - 40天趋于上升。出生后0至20小时皮质醇产量水平显著高于皮质酮产量水平，而从第12 - 14天起前者显著低于后者。出生后0至8小时皮质酮与总皮质类固醇（皮质醇 + 皮质酮）的比率非常低（0.28±0.04），但在第35 - 40天逐渐上升，此时为0.95±0.01。本研究结果表明，在兔出生后的早期生活中，皮质醇是肾上腺细胞的主要产物。
