Roebuck M M, Jones C T, Robinson J S, Mitchell M D, Thorburn G D
Acta Endocrinol (Copenh). 1984 Apr;105(4):545-51. doi: 10.1530/acta.0.1050545.
Cells from adrenal glands of 140-160 days foetal, neonatal and infant Rhesus monkeys (Macaca mulatta) were prepared, by collagenase digestion and incubated with 10 pg-16 ng/ml ACTH. The production of cortisol, androstenedione, corticosterone, progesterone and prostaglandins was measured. The cortisol production in the 140 day and 160 day foetuses and in the neonatal adrenal cells was comparable. It was 2-fold higher in adrenal cells of 6 month infant monkeys. In all the groups there was an increasing production of cortisol with increasing ACTH concentration, and a response to low physiological concentrations of ACTH. The androstenedione production was significantly greater in the 160 day foetuses than in either those of 140 days or of the neonate which demonstrated a poor response to increasing ACTH concentrations. It responded well to increasing ACTH in adrenal cells from 6 month infant monkeys. Corticosterone output was 1/10th of cortisol with only the 140 day foetuses showing an increase in production with increasing ACTH concentrations. The results demonstrate that cells of the primate foetal adrenal gland are not inherently unresponsive to ACTH stimulation as regards cortisol production, which per/micrograms DNA does not appear to change over the last 25 days before term.
制备140 - 160日龄胎儿、新生儿和幼年恒河猴（猕猴）肾上腺细胞，采用胶原酶消化法，并与10 pg - 16 ng/ml促肾上腺皮质激素（ACTH）一起孵育。检测皮质醇、雄烯二酮、皮质酮、孕酮和前列腺素的生成量。140日龄和160日龄胎儿以及新生儿肾上腺细胞的皮质醇生成量相当。6个月龄幼年猴的肾上腺细胞中皮质醇生成量高出2倍。在所有组中，随着ACTH浓度升高，皮质醇生成量增加，且对低生理浓度的ACTH有反应。160日龄胎儿的雄烯二酮生成量显著高于140日龄胎儿或新生儿，后两者对ACTH浓度升高的反应较差。6个月龄幼年猴肾上腺细胞中的雄烯二酮对ACTH浓度升高反应良好。皮质酮产量是皮质醇的1/10，只有140日龄胎儿的皮质酮产量随ACTH浓度升高而增加。结果表明，灵长类胎儿肾上腺细胞在皮质醇生成方面并非天生对ACTH刺激无反应，在足月前的最后25天里，每微克DNA的皮质醇生成量似乎没有变化。
