Immunology and Cell Biology Core Laboratory, Catholic Research Institutes of Medical Science,The Catholic University of Korea, Seoul, Korea.
Evid Based Complement Alternat Med. 2009 Dec;6(4):457-64. doi: 10.1093/ecam/nem158. Epub 2007 Oct 22.
In the present study, we investigated the effects of Acori graminei rhizoma (AGR) on learning and memory for the Morris water maze task and on the central cholinergic system of the rats with excitotoxic medial septum (MS) lesion. On the water maze test, the rats were trained to find a platform that was in a fixed position during 6 days and then they received a 60 s probe trial in which the platform was removed from the pool on the 7th day. Ibotenic lesioning of the MS impaired the performance on the maze test and it caused degeneration of choline acetyltransferase and acetylcholine esterase in the hippocampus, which are markers of the central cholinergic system. Daily administrations of AGR (100 mg kg(-1), i.p.) for 21 consecutive days produced reversals of the ibotenic acid-induced deficit in learning and memory. These treatments also reduced the loss of cholinergic immunoreactivity in the hippocampus that was induced by ibotenic acid. These results demonstrated that AGR ameliorated learning and memory deficits through their effects on the central nervous system, and neuroprotection was partly evaluated through the effect of AGR on the cholinergic system. Our studies suggest that AGR can possibly be used as treatment for Alzheimer's disease.
在本研究中，我们研究了石菖蒲对学习和记忆的影响，以及对兴奋性中隔（MS）损伤大鼠中枢胆碱能系统的影响。在水迷宫测试中，大鼠被训练在 6 天内找到一个固定位置的平台，然后在第 7 天的 60 秒探测试验中移除平台。MS 的异博定损伤损害了迷宫测试的表现，并导致海马体中的胆碱乙酰转移酶和乙酰胆碱酯酶退化，这是中枢胆碱能系统的标志物。连续 21 天每天给予石菖蒲（100mg/kg，腹腔注射）可逆转异博定诱导的学习和记忆缺陷。这些治疗方法还减少了异博定诱导的海马体胆碱能免疫反应的丧失。这些结果表明，石菖蒲通过对中枢神经系统的作用改善了学习和记忆缺陷，并且通过石菖蒲对胆碱能系统的作用部分评估了神经保护作用。我们的研究表明，石菖蒲可能可用于治疗阿尔茨海默病。
