School of Pharmaceutical Sciences, Southern Medical University, 1838# North Guangzhou Road, Guangzhou 510515, China.
J Chromatogr A. 2010 Jan 1;1217(1):175-8. doi: 10.1016/j.chroma.2009.11.038. Epub 2009 Nov 18.
An analytical strategy micelle to trapping solution stacking (MSS) was developed in acidic buffer in micellar electrokinetic chromatography (MEKC). The stacking mechanism is based on the transport, release, capturing of molecules bound to micelle carriers that are made to collapse into trapping solution (TS) to serve as the medium to contain and stacking the analytes. Tetrandrine and fangchinoline were selected as model mixture using sodium dodecyl sulfate (SDS) micelles as carrier to demonstrate this stacking method. The experiments by MSS-MEKC were carried out and further compared with those by normal MEKC. The results reveal that 113-123-fold improvements in the detection sensitivity was obtained for the analytes, and separation and determination of tetrandrine and fangchinoline in Stephaniae tetrandrae S. Moore and Fengtongan capsules were finished under optimum conditions using the sample matrix containing 8.0mM SDS and TS containing 50mM H(3)PO(4)-55% (v/v) ethanol.
建立了在酸性缓冲液中胶束电动色谱（MEKC）中的胶束增敏在线浓缩进样（MSS）分析策略。该浓缩机制基于分子与胶束载体的结合物的传输、释放和捕获，使胶束载体坍塌进入浓缩溶液（TS）中，作为包含和浓缩分析物的介质。使用十二烷基硫酸钠（SDS）胶束作为载体，选择汉防己甲素和粉防己碱作为模型混合物来证明这种浓缩方法。通过 MSS-MEKC 进行了实验，并与常规 MEKC 进行了进一步比较。结果表明，分析物的检测灵敏度提高了 113-123 倍，在最佳条件下，使用含有 8.0mM SDS 的样品基质和含有 50mM H（3）PO（4）-55%（v/v）乙醇的 TS，完成了粉防己和防己通胶囊中汉防己甲素和粉防己碱的分离和测定。
