Papadopoulos Giorgos L, Reczko Martin, Simossis Victor A, Sethupathy Praveen, Hatzigeorgiou Artemis G
Institute of Molecular Oncology, Biomedical Sciences Research Center Alexander Fleming, 166 72 Varkiza, Synaptic Ltd, 711 10 Heraklion, Greece.
Nucleic Acids Res. 2009 Jan;37(Database issue):D155-8. doi: 10.1093/nar/gkn809. Epub 2008 Oct 27.
TarBase5.0 is a database which houses a manually curated collection of experimentally supported microRNA (miRNA) targets in several animal species of central scientific interest, plants and viruses. MiRNAs are small non-coding RNA molecules that exhibit an inhibitory effect on gene expression, interfering with the stability and translational efficiency of the targeted mature messenger RNAs. Even though several computational programs exist to predict miRNA targets, there is a need for a comprehensive collection and description of miRNA targets with experimental support. Here we introduce a substantially extended version of this resource. The current version includes more than 1300 experimentally supported targets. Each target site is described by the miRNA that binds it, the gene in which it occurs, the nature of the experiments that were conducted to test it, the sufficiency of the site to induce translational repression and/or cleavage, and the paper from which all these data were extracted. Additionally, the database is functionally linked to several other relevant and useful databases such as Ensembl, Hugo, UCSC and SwissProt. The TarBase5.0 database can be queried or downloaded from http://microrna.gr/tarbase.
TarBase5.0是一个数据库，它手动整理了多个具有核心科学价值的动物物种、植物和病毒中经实验支持的微小RNA（miRNA）靶标的集合。miRNA是一类小的非编码RNA分子，对基因表达具有抑制作用，会干扰靶向成熟信使RNA的稳定性和翻译效率。尽管存在多种用于预测miRNA靶标的计算程序，但仍需要一个经实验支持的miRNA靶标的综合集合和描述。在此，我们介绍该资源的一个大幅扩展版本。当前版本包含1300多个经实验支持的靶标。每个靶位点都由与之结合的miRNA、其所在的基因、用于测试它的实验性质、该位点诱导翻译抑制和/或切割的充分性，以及提取所有这些数据的论文进行描述。此外，该数据库在功能上与其他几个相关且有用的数据库相链接，如Hugo、UCSC和SwissProt。可从http://microrna.gr/tarbase查询或下载TarBase5.0数据库。
